凋亡相关基因p53、bcl-2在乳腺导管非典型增生中表达的研究

为观察凋亡相关基因-p53,bcl-2在乳腺导管非典型增生及乳腺癌中的表达，探讨其与乳腺癌组织发生的关系。本实验应用原位杂交方法检测凋亡相关基因p53,bcl-2mRNA，应用免疫组织化学方法检测p53蛋白在44例乳腺导管非典型增生组织中的表达，并与6例乳腺导管单纯性增生及26例乳腺癌对比分析，实验结果为，p53mRNA在乳腺导管单纯性增生组织中呈较强表达（66.7%)，在乳腺导自欺欺人这非典型增生组织中阳性表达为40%（轻度；55.6%，中度：41.7%，重度；26.1%)。在癌组织中的表达率为19.2%(导管内癌：21.4%，浸润性导管癌：16.7%),p53蛋白在导管单纯性增生组无表达，在导管非典型增生组阳性表达为24%（轻度：11.1%，中度；25%，重度；34.8%)。在癌组织中的阳性表达为38.4%(导管内癌：35.7%，浸润性导管癌：41.7%),bcl-2mRNA在单纯性增生组无表达，在非典型增生组中阳性表达为轻度：11.1%，中度：16.7%，重度：39.1%，在乳腺癌组织中阳性表达为导管内癌；78.6%，浸润性导管癌83.3%。实验结果表明，在乳腺导管重度非典型增生组织中可检测到p53基因有较高的表达缺失，突变及bcl-2mRNA过表达。

EXPRESSION OF APOPTOSIS RELATED GENES P53 BCL-2 IN MAMMARY ATYPICAL DUCTAL HYPERPLASIA

The aim of the study was to investigate the expression of apoptosis related genes p53?bcl 2 in mammary atypical ductal hyperplasia and breast carcinoma and to explore the relationship between the gene expression of p53?bcl 2 and the progression of breast carcinoma and activity of telomerase In 44 mammary atypical ductal hyperplasia cases,apoptosis related genes p53?bcl 2 were detected by in situ hybridization and expression of p53 protein was detected by immunohistochemistry method,and compared with that of the 6 simple hyperplasia and 26 carcinoma of breast The expression of p53 mRNA was higher in simple hyperplasia(66 7%),40% in atypical ductal hyperplasia(mild 55 6%,medium 41 7%,severe 26 1%)and 19 2% in carcinoma (intraductal carcinoma 21 4%,invasive ductual carcinoma 16 7%) The expression of p53 protein was negative in simple hyperplasia,24% in atypical ductal hyperplasia(mild 11 1%,medium 25%,severe 34 8%),38 5% in carcinoma(intraductal carcinoma 35 7%,invasive ductal carcinoma 41 7%) The expression of bcl 2 mRNA was negative in simple hyperplasia,11 1% in mild, 16 7% in medium and 39 1% in severe atypical ductal hyperplasia, 78 6% in intraductal carcinoma and 83 3% in invasive ductal carcinoma The results showed that the loss of expression and mutation of p53 gene and the excessive expression of bcl 2 mRNA were detected in severe atypical ductal hyperplasia.