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| 缺血再灌注对小鼠肠神经丛nNOS 和iNOS表达的影响 | |
| 引用本文： | 梅盛平,蒋芬,胡伊乐,张艳,董大翠.缺血再灌注对小鼠肠神经丛nNOS 和iNOS表达的影响[J].中国组织化学与细胞化学杂志,2006,15(5):542-546. |
| 作者姓名： | 梅盛平 蒋芬 胡伊乐 张艳 董大翠 |
| 作者单位： | 1. 湖北职业技术学院医学院人体解剖学教研室,孝感,432000 2. 华中科技大学同济医学院解剖学系,武汉,430030 3. 河南科技大学医学院解剖学教研室,洛阳,41003 |
| 摘 要： | 目的观察缺血再灌注后小鼠回肠神经型一氧化氮合酶(neuron alnitric oxide synthase,nNOS)和诱导型一氧化氮合酶(induciblenitric oxide synthase,iNOS)的表达,探讨肠缺血再灌注损伤(ischemia-reperfusion injury,IRI)的发生机制。方法采用小鼠肠系膜上动脉缺血再灌注模型,根据不同再灌注时间对小鼠随机分1d组、3d组、5d组、7d组、对照组和假手术组,用SP法检测小鼠回肠nNOS和iNOS的表达情况。结果与对照组和假手术组相比较,nNOS在再灌注1d后开始在肌间神经丛持续高表达(P<0.01);而iNOS在再灌注3d后开始在肌间神经丛持续高表达(P<0.05)。结论nNOS和iNOS在肠缺血再灌注后的表达增强,提示一氧化氮及一氧化氮合酶与肠神经节细胞在缺血再灌注中的损伤有着密切关系。 |
| 关 键 词： | 一氧化氮合酶 一氧化氮 缺血再灌注 回肠 小鼠 |
| 收稿时间： | 01 10 2006 12:00AM |
| 修稿时间： | 03 8 2006 12:00AM |
EFFECT OF ISCHEMIA-REPERFUSION ON THE EXPRESSION OF nNOS AND iNOS IN MOUSE INTESTINAL NERVE PLEXUS |
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| Mei Shengping,Jiang Fen,Hu Yile,Zhangyan,Dong Dacui.EFFECT OF ISCHEMIA-REPERFUSION ON THE EXPRESSION OF nNOS AND iNOS IN MOUSE INTESTINAL NERVE PLEXUS[J].Chinese Journal of Histochemistry and Cytochemistry,2006,15(5):542-546. | |
| Authors: | Mei Shengping Jiang Fen Hu Yile Zhangyan Dong Dacui |
| Institution: | 1.Department of Anatomy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030; 2.Department of Human Anatomy, Medical College of Hubei Vocational-Technical College, Xiaogan 432000; 3.Department of Anatomy, Medical College of Henan University of Science and Technology, Luoyang 471003, China |
| Abstract: | Objective To investigate the mechanism of ischemia-reperfusion injury by observing the expression of neuronal nitric oxide synthase(nNOS)and inducible nitric oxide synthase(iNOS)in mouse ileum after ischemia-reperfusion.Methods In a model of superior mesenteric artery occlusion followed by reperfusion in mice,36 mice were randomly divided into groups according to the time of reperfusion:1d group,3d group,5d group,7d group,control group and pseudo-operation group.Then,the expression of nNOS and iNOS was observed by immunohistochemical SP method.Results Compared with control group and pseudo-operation group,nNOS showed high immunoreactivity since 1d reperfusion following 45 min ischemia(P<0.01),but iNOS kept high immunoreactivity since 3d reperfusion following 45 min ischemia(P<0.05).Conclusion The upregulated expression of nNOS and iNOS in mouse ileum after ischemia-reperfusion suggests that NO and NOS are associated with the injury of the intestinal nerve plexus. |
| Keywords: | Nitric oxide synthase Nitric oxide Ischemia-reperfusion Ileum Mouse |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |