Nogo—p4与NgR结合抑制神经干细胞分化为双极形星形胶质细胞的突起生长

目的观察Nogo—p4是否通过与NgR结合的途径对大鼠脊髓来源神经干细胞分化形成双极形星形胶质细胞突起长度产生抑制。方法取4只出生24h内的Wistar大鼠，悬浮培养法培养大鼠脊髓来源的神经干细胞。把神经干细胞分为A、B、C、D四组，A组加入血清，B组加入血清和Nogo—p4，C组神经干细胞经RNA干扰沉默NgR基因后加入血清分化，D组神经干细胞经RNA干扰沉默NgR基因后加入血清和Nogo—p4。分化第7d，GFAP抗体标记星形胶质细胞，使用Image—ProPlus5．0软件测量双极形星形胶质细胞突起长度。结果神经干细胞分化第7d，四组均可形成双极形星形胶质细胞。B组中双极形星形胶质细胞的突起长度明显短于其它各组。A、C、D组中双极形星形胶质细胞的突起长度没有显著差异。结论Nogo—p4经与NgR结合途径显著抑制脊髓来源神经干细胞分化成的双极形星形胶质细胞的突起生长。

Nogo-p4 in combination with NgR inhibit the process growth of bipolar astrocytes differentiated from neural stem cells

Objective To observe the effect of Nogo-p4 on process growth of bipolar astrocyte differ- entiated from neural stem cells （NSCs） with and without the silenced NgR gene. Methods Neurospheres were collected by suspension culture of cells obtained from spinal cords of 4 wistar rats born in 24 hours. Immunofluorescence method was used to identify NSCs and detect the expression of GFAP after NSCs dif- ferentiated. The NSCs were divided into groups A, B, C and D. FBS was added to group A to initiate glial differentiation; FBSq-Nogo-p4 was added to group B; in group C NSCs were transfected by siRNA to knockdown NgR before they differentiated; FBSq-Nogo-p4 was added to group D after siRNA transfection and silencing of the NgR gene. The process length of bipolar astrocytes was measured by Image-Pro Plus 5.0 software. Results Seven days after differentiation, bipolar astrocytes were observed in each group. The length of the processes on bipolar astroeytes in group B was significantly shorter than that in other groups. Conclusion In combination with NgR, Nogo-p4 inhibits the process growth of bipolar astrocytes differenti- ated from NSCs derived from spinal cords of rats.