c-Met在人乳腺癌组织中的表达及其与明胶酶的关系

目的研究c—Met蛋白在人乳腺癌组织中的表达及其临床意义，探讨其与乳腺癌明胶酶（MMP-2和MMP-9）关系。方法应用免疫组织化学方法检测86例乳腺癌组织c—Met蛋白的表达情况，分析它们与患者临床病理特征和预后的关系；使用siRNA技术特异性下调乳腺癌细胞内源性c—Met后，westernblot方法检测乳腺癌细胞MMP-2和MMP-9表达水平。结果人乳腺癌c—Met蛋白表达阳性率为58．1％，其表达与肿瘤淋巴结转移和临床分期均呈显著正相关（P〈O．01），与患者总生存期和无复发生存期均呈负相关（P〈O．01）；相关性分析显示：乳腺癌c—Met和MMP-2及MMP-9表达均呈显著正相关（r=0．314和0．322，P〈O．01）；使用siRNA特异性下调乳腺癌MDA—M13-231细胞c—Met表达后，MMP-2和MMP-9表达也显著降低。结论乳腺癌c—Met表达状况与侵袭转移密切相关，其功能可能是通过调控MMP-2和MMP-9表达而发挥作用。

Clinical impact of c-Met and its relationship with gelatinase in human breast cancer

Objective To investigate the clinical significance of c-Met in human breast cancer, and the relationship between c-Met and gelatinase （MMP-2 and MMP-9）. Methods The expression of c-Met pro- tein was detected in 86 cases of breast cancer by immunohistochemistry. The expression of MMP-2 and MMP-9 in breast cancer cells was examined after transfection with c-Met siRNA by western blot assay. Results The positive rate of c-Met protein was 58.1~ in breast cancer, which was significantly correlated to lymph node metastasis, clinical stage and patient survival（P~0. 01）. A significant correlation was found between the expression of c-Met and gelatinase protein in breast cancer（r=0. 314 and 0. 322,P〈0. 01）. The expression of gelatinase was significantly downregulated by transfeetion with c-Met siRNA in MDA-MB-231 cells, compared with that of controls. Conclusion The c-Met plays an important role in breast cancer invasion and metastasis by partly regulating the expression of gelatinase.