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| SIAH2与P-ERK在乳腺癌中的表达及其临床意义 | |
| 引用本文： | 孟媛,董经宇,甄娟,孙洁,宋敏,李柏林.SIAH2与P-ERK在乳腺癌中的表达及其临床意义[J].中国组织化学与细胞化学杂志,2011,20(3):220-226. |
| 作者姓名： | 孟媛 董经宇 甄娟 孙洁 宋敏 李柏林 |
| 作者单位： | 1. 中国医科大学基础医学院病理学教研室 2. 中国医科大学92期七年制,辽宁,沈阳,110001 3. 中国医科大学基础医学院病理学教研室及中国医科大学附属第一医院病理科 4. 中国医科大学基础医学院化学教研室 |
| 基金项目： | 辽宁省教育厅科研基金资助项目 |
| 摘 要： | 目的观察人乳腺细胞系及乳腺组织中SIAH2和P-ERK表达的变化,探讨乳腺癌中SIAH2与P-ERK的关联。方法应用免疫组织化学检测140例乳腺石蜡包埋组织中SIAH2和P-ERK的表达,Western blot检测人乳腺细胞系和23例乳腺浸润性导管癌及癌旁正常乳腺组织中SIAH2和P-ERK的蛋白表达情况,人乳腺癌细胞系表达SIAH2-siRNA后,P-ERK蛋白表达情况。结果乳腺癌中SIAH2和P-ERK阳性率与组织学分级相关(P<0.05),SIAH2和P-ERK呈正相关性。人乳腺癌细胞系以及乳腺癌组织中SIAH2和P-ERK蛋白表达量明显高于人乳腺正常上皮细胞系与癌旁正常乳腺组织中SIAH2和P-ERK蛋白表达量(P<0.05);表达SIAH2-siRNA的乳腺癌细胞系与对照组相比,P-ERK蛋白表达明显减少(P<0.05)。结论 SIAH2、P-ERK过表达与乳腺癌的组织学分级有关。在乳腺癌细胞系中抑制SIAH2表达后,P-ERK表达也减少,因此抑制SIAH2可以抑制ERK通路。 |
| 关 键 词： | SIAH2 P-ERK 乳腺癌 临床病理特征 干扰 |
Expression of SIAH2 and P-ERK in breast cancer and its clinical significances |
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| Meng Yuan,Dong Jingyu,Zhen Juan,Sun Jie,Song Min,Li Bailin.Expression of SIAH2 and P-ERK in breast cancer and its clinical significances[J].Chinese Journal of Histochemistry and Cytochemistry,2011,20(3):220-226. | |
| Authors: | Meng Yuan Dong Jingyu Zhen Juan Sun Jie Song Min Li Bailin |
| Institution: | Meng Yuan,Dong Jingyu3,Zhen Juan,Sun Jie,Song Min1,Li Bailin2(University,Shenyang 110001,China,2 Department of Chemisry,College of Basic Medical Sciences,china Medical University,3 92K in 7-system-class,China MedicalUniversity,China) |
| Abstract: | Objective To investigate the expression of SIAH2 and P-ERK in human breast cell lines and breast tissues and their correlation in the malignant progression of breast carcinoma.Methods The expression of SIAH2 and P-ERK was examined in 140 paraffin-embeded specimens using immunohistochemical staining.Human breast cells and 23 cases of breast cancer and pairing non-neoplastic tissue were subjected to Western blot for SIAH2 and P-ERK proteins.The expression of P-ERK in SIAH2 siRNA transfected cells was examined... |
| Keywords: | SIAH2 P-ERK |
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