人参皂甙对老龄大鼠海马结构BDNF及Trk B蛋白表达的影响

目的探讨老龄大鼠海马结构BDNF及Trk B蛋白表达的老龄性变化,同时对比观察人参皂甙对其改变的影响。方法雌性Wistar大鼠39只,分为青年组、老龄组、给药组(第17个月始饲以人参皂甙至27月龄)。采用免疫组化及Western blot方法对海马结构BDNF反应产物及Trk B蛋白进行定性、定量分析。结果老龄组CA3、CA1区BDNF含量分别较青年组下降13.3%、10.4%(P<0.05);其给药组和老龄组相比变化不大(P>0.05)。齿状回从青年到老年变化不明显(P>0.05),但给药组比老龄组增加16.7%(P<0.01)。老龄组海马结构Trk B蛋白表达较青年组下调了99.7%;给药组较老龄组上调了78.5%(P<0.01)。结论老龄组海马结构BDNF及Trk B蛋白表达较青年组明显降低,而人参皂甙可明显上调齿状回BDNF含量和海马结构Trk B蛋白的表达。

EFFECTS OF GINSENOSIDES ON BDNF AND Trk B PROTEIN EXPRESSION IN HIPPOCAMPAL FORMATION OF AGED RATS

Objective To investigate the age-associated changes of BDNF and Trk B protein expression in hippocampal formation and the effects of ginsenosides(GS) on their expression.Methods 39 Wistar rats were divided randomly into 3 groups: young, aged and GS.The rats of the GS group were fed with GS from 17 to 27 months.Immunohistochemistry and Western blotting were used to qualify and quantitate BDNF and Trk B protein expression in hippocampal formation.Results BDNF levels in CA3 and CA1 in aged hippocampal formation decreased 13.3% and 10.4% respectively(P<0.05),while no difference was found between the aged and the GS group.In contrast,there was no obvious age-related difference in dentate gyrus(P>0.05),but we found BDNF expression in dentate gyrus increased 16.7%(P<0.01) in the GS group compared with that of the aged group.Trk B protein expression in aged hippocampal formation decreased 99.7% compared with that of the young group,and its expression in GS group increased 78.5% compared with that of the aged group(P<0.01).Conclusion BDNF and Trk B expression in hippocampal formation of aged rats decreases significantly,and GS can increase their expression.