| 大鼠实验性胃溃疡期间下颌下腺TFF3基因的变化 |
| |
| 引用本文： | 吴靖芳,顾彩霞,张静,郑慧娥,王浩宇,张江兰.大鼠实验性胃溃疡期间下颌下腺TFF3基因的变化[J].中国组织化学与细胞化学杂志,2010,19(1):26-31. |
| |
| 作者姓名： | 吴靖芳 顾彩霞 张静 郑慧娥 王浩宇 张江兰 |
| |
| 作者单位： | 1. 河北北方学院组织学与胚胎学教研室,张家口,075000
2. 河北北方学院06级临床本科,张家口,075000 |
| |
| 基金项目： | 河北省自然科学基金(C2007000906) |
| |
| 摘 要： | 目的研究下颌下腺肠三叶因子(intestinal trefoil peptide,ITF即TFF3)基因在大鼠实验性胃溃疡自愈过程的变化,探讨其与胃溃疡自愈的关系。方法通过胃窦前壁黏膜下注射冰乙酸制备大鼠胃溃疡模型:⑴用免疫组织化学SABC法和RT-PCR检测42只溃疡组,21只盐水组,及6只正常组大鼠下颌下腺组织中TFF3肽和TFF3mRNA的表达情况。结果(1)免疫组化显示:溃疡组大鼠下颌下腺的TFF3肽主要表达于导管系统上皮,如闰管、颗粒曲管(granular convoluted tubule,GCT)以及纹状管、小叶间导管上皮细胞、黏液腺泡细胞也有少量分布,浆液腺泡细胞呈阴性。溃疡组手术后第1d时,下颌下腺TFF3表达明显强于盐水组和正常组(P0.01)。术后第2d,积分光密度明显低于1d溃疡组(P0.05),4、6d积分光密度逐渐增强并高于对照组(P0.05),到术后第10d达高峰(P0.01),23d积分光密度仍高于对照组(P0.05)。(2)RT-PCR显示:溃疡1、2、4、6、10、14、23dTFF3/GAPDH光密度比值分别为1.42±0.10,1.18±0.13,1.29±0.15,1.24±0.17,1.57±0.19,1.25±0.14,1.13±0.16明显高于相应盐水对照组的TFF3/GAP-DH光密度比值(P0.01)。结论大鼠胃溃疡时期,下颌下腺TFF3基因上调,推测下颌下腺TFF3通过外分泌或内分泌参与胃溃疡愈合过程
|
| 关 键 词： | 肠三叶因子 胃溃疡 下颌下腺 大鼠 免疫组织化学 RT-PCR |
CHANGE OF TREFOIL FACTOR 3 GENE IN EXPERIMENTAL GASTRIC ULCER IN RATS OF SUBMAXIL ARY GLAND |
| |
| Wu Jingfang,Gu Caixia,Zhang Jing,Zheng Huie,Wang Haoyu,Zhang Jianglan.CHANGE OF TREFOIL FACTOR 3 GENE IN EXPERIMENTAL GASTRIC ULCER IN RATS OF SUBMAXIL ARY GLAND[J].Chinese Journal of Histochemistry and Cytochemistry,2010,19(1):26-31. |
| |
| Authors: | Wu Jingfang Gu Caixia Zhang Jing Zheng Huie Wang Haoyu Zhang Jianglan |
| |
| Institution: | Department of Histology;1 06 grade clinical student;Hebei Northern University;Zhangjiakou;075000;China |
| |
| Abstract: | Objective To explore the expression of trefoil factor3 (TFF3) gene in submaxilary glands during the self-healing of experimental gastric ulcer in rats. Methods Gastic ulcer was induced by injecting acetic acid to the mucosa of paries anterior gastricus. The expressions of TFF3 peptide and TFF3mRNA in 42 rats with gastric ulcer, 21 control rats and 6 normal rats were detected respectively by immunohistochemical SABC method and RT-PCR. Results (1)TFF3 immunoreactive cells were mainly located in the epithelium... |
| |
| Keywords: | Trefoil factor3 Gastric ulcer Submaxilary gland Rat Immunhistochemistry RT-PCR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
