| COX-2和P-STAT_3、P-STAT_5在肝癌细胞株SMMC-7721中的表达及意义 |
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| 引用本文： | 赵军艳,郑艳敏,李轩.COX-2和P-STAT_3、P-STAT_5在肝癌细胞株SMMC-7721中的表达及意义[J].中国组织化学与细胞化学杂志,2011,20(2):144-147. |
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| 作者姓名： | 赵军艳 郑艳敏 李轩 |
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| 作者单位： | 武警医学院附属医院消化内科,天津,300162 |
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| 基金项目： | 武警医学院博士启动金课题(WBS200814) |
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| 摘 要： | 目的观察COX-2和P-STAT3、P-STAT5在人肝癌细胞株SMMC-7721中的表达及其相互关系,探讨环氧合酶2表达与JAK/STAT信号转导通路之间的关系。方法培养人肝癌SMMC-7721细胞,应用免疫组化法检测COX-2抑制剂塞来昔布处理人肝癌细胞株SMMC-7721前后COX-2、P-STAT3、P-STAT5表达的变化。结果COX-2、P-STAT3、P-STAT5在人肝癌细胞株SMMC-7721均呈高表达,并且COX-2与P-STAT3、P-STAT5的表达呈显著正相关;应用COX-2抑制剂塞来昔布后COX-2、P-STAT3、P-STAT5的表达均显著降低,用药前后相比差异有显著性(P<0.01);但用药后COX-2与P-STAT3、P-STAT5的表达无显著相关性。结论COX-2和JAK/STAT通路有密切联系,抑制COX-2的过度表达可能影响JAK/STAT细胞信号转导通路的活性。
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| 关 键 词： | 环氧合酶-2 塞来昔布 肝细胞癌 P-STAT3 P-STAT5 |
The expression and significance of COX-2,P-STAT_3 and P-STAT_5 in hepatoma cells |
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| Zhao Junyan,Zheng Yanmin,Li Xuan.The expression and significance of COX-2,P-STAT_3 and P-STAT_5 in hepatoma cells[J].Chinese Journal of Histochemistry and Cytochemistry,2011,20(2):144-147. |
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| Authors: | Zhao Junyan Zheng Yanmin Li Xuan |
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| Institution: | Zhao Junyan,Zheng Yanmin,Li Xuan(Department of Gastroenterology,Affiliated Hospital of the Medical College of Chinese People's Armed Police Force,Tianjin 300162,China) |
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| Abstract: | Objective To study the expression and correlation of COX-2,P-STAT3 and P-STAT5 in hepatoma cells,and to observe the correlation between COX2 and JAK/STAT signaling pathway.Methods The changes of COX-2,P-STAT3 and P-STAT5 expressions were investigated with immunocytochemistry.Results The expressions of COX-2,P-STAT3,and P-STAT5 in HCC were highly,and there were significantly positive correlations of COX2 with P-STAT3 and P-STAT5.The expressions of COX2,P-STAT3 and P-STAT5 were significantly lower after treat... |
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| Keywords: | COX-2 Celecoxib Hepatoma cells P-STAT3 P-STAT5 |
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