上皮性卵巢癌组织中P-AKT、P-gp的表达及其临床病理意义

目的探讨卵巢上皮癌组织中P-AKT(或称磷酸化蛋白激酶B,phosphorylated protein kinase B)和P-gp(P-glycoprotein)的表达及其与临床病理因素之间的关系。方法采用免疫组化法检测60例卵巢上皮癌组织、30例卵巢良性肿瘤组织和30例正常卵巢组织中P-AKT、P-gp的表达。结果(1)P-AKT、P-gp在上皮性卵巢癌中的阳性表达率(61.7%、50.0%)高于其在卵巢良性肿瘤组织和正常卵巢组织中的表达(13.3%、1.7%;6.7%、0),P0.05。(2)P-AKT和P-gp的异常表达均与卵巢癌的FIGO分期相关(P0.05),与组织学类型、病理分级无关。(3)P-AKT和P-gp蛋白的过表达呈显著正相关性(r=0.26,P0.05)。(4)P-AKT、P-gp阳性组的复发率(51.4%、73.3%)高于其阴性组的复发率(26.9%、30.8%),P0.05。P-AKT、P-gp阳性组的生存率(62.2%、56.7%)低于其阴性组的生存率(91.3%、90.0%)P0.05。结论:P-AKT、P-gp与上皮性卵巢癌的发展及预后有关。P-AKT可能通过作用于其下游的P-gp蛋白促进卵巢癌细胞的恶性转化及对化疗药物的耐药。

EXPRESSIONS AND THEIR CLINICAL SIGNIFICANCE OF P-AKT AND P-GP IN EPITHELIAL OVARIAN CANCER TISSUES

Objective To investigate the expression of P-AKT（phosphorylated protein kinase B）and P-gp（P-glycoprotein） in epithelial ovarian cancer（EOC） and the clinical significance.Methods Immunohistochemical staining was used to detect the expression of P-AKT and P-gp in 60 cases of EOC,30 cases of benign epithelial ovarian tumor and 30 cases of normal ovarian tissue.Results（1） The positive expressions of P-AKT and P-gp were significantly higher in EOC（61.7%、50.0%） than in the normal ovary group（6.7%,0） and the benign tumor group（13.3%,1.7%,all P〈0.05）.（2） The expressions of P-AKT and P-gp in EOC were both associated with FIGO stage,but not with pathologic stage and histological type.（3） Overexpressions of P-AKT and P-gp were positively correlated（r=0.26,P〈0.05）.（4） The recurrence rates were signigicantly higher in the positive P-AKT and P-gp groups（51.4%,73.3%） than in the negative groups respectively（26.9%、30.8%,all P〈0.05）.The survival rates in the positive groups of P-AKT and P-gp（62.2%,56.7%） were lower than in the negative groups（91.3%,90.0%） respectively（all P〈0.05）.Conclusion P-AKT and P-gp may play important roles in the development and prognosis of epithelial ovarian cancer.P-AKT may up-regulate the expression of P-gp which may promote the malignant transformation of ovarian cancer cells and resistance to chemotherapeutics.