| 波形蛋白在实验性肾间质纤维化上皮-间充质转分化中的表达及意义 |
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| 引用本文： | 周娟,张悦,陆海英,刘煜敏.波形蛋白在实验性肾间质纤维化上皮-间充质转分化中的表达及意义[J].中国组织化学与细胞化学杂志,2009,18(1). |
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| 作者姓名： | 周娟 张悦 陆海英 刘煜敏 |
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| 作者单位： | 1. 上海中医药大学科技实验中心,上海,201203
2. 上海中医药大学护理学院,上海,201203 |
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| 摘 要： | 目的明确肾脏纤维化中波形蛋白表达在上皮-间充质转分化观察中的意义。方法雄性SD大鼠12只,随机分成假手术组和模型组,模型组行单侧输尿管梗阻术。造模后14天处死大鼠,分别采用免疫组织化学染色和Western印迹法对梗阻侧肾组织波形蛋白和α平滑肌肌动蛋白作定性和定量检测。并通过体外实验用TGF-β1刺激诱导人近端小管上皮细胞株(HK-2)发生上皮-间充质转分化。采用间接免疫荧光法对E-钙粘蛋白和波形蛋白进行染色,采用激光共聚焦显微镜观察细胞形态改变,并用蛋白印迹法定量检测HK-2细胞波形蛋白和α平滑肌肌动蛋白表达水平。结果萎缩和扩张的肾小管上皮细胞出现波形蛋白和α平滑肌肌动蛋白表达,肾组织中波形蛋白和α平滑肌肌动蛋白表达量显著增加;正常的HK-2细胞,细胞形态为不规则圆形,TGF-β1刺激后细胞伸展成长梭形;细胞α平滑肌肌动蛋白表达水平显著增加,波形蛋白表达水平无显著变化。结论体内实验研究上皮-间充质转分化,将波形蛋白作为间充质细胞标志物具有较好的参考价值,而体外研究中,其标志作用尚存在争议。
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| 关 键 词： | 上皮-间充质转分化 波形蛋白 单侧输尿管梗阻 转化生长因子-β1 |
EXPRESSION AND SIGNIFICANCE OF VIMENTIN IN EPITHELIAL-MESENCHYMAL TRANSDIFFERENTIATION IN KIDNEY FIBROSIS |
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| Zhou Juan,Zhang Yue,Lu Haiying,Liu Yumin.EXPRESSION AND SIGNIFICANCE OF VIMENTIN IN EPITHELIAL-MESENCHYMAL TRANSDIFFERENTIATION IN KIDNEY FIBROSIS[J].Chinese Journal of Histochemistry and Cytochemistry,2009,18(1). |
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| Authors: | Zhou Juan Zhang Yue Lu Haiying Liu Yumin |
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| Institution: | Scientific Experimental Center of Shanghai TCM University;1Nursing College of Shanghai TCM University;Shanghai 201203;China |
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| Abstract: | Objective To clarify the expression and significance of vimentin in epithelial-mesenchymal transdifferentiation in kidney fibrosis.Methods In vivo: 12 male SD rats were randomly divided into 2 groups, 6 in sham-operated group and the other 6 in the model group with unilateral ureteral obstruction. All the rats were killed at day 14, immunohistochemistry and Western blot were applied respectively for qualitative and quantitative test of vimentin and α-smooth muscle actin (α-SMA) in kidney. In vitro: The norm... |
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| Keywords: | Epithelial-mesenchymal transdifferentiation Vimentin Unilateral ureteral obstruction TGF-β1 |
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