| 高糖刺激下大鼠肾小球系膜细胞CTGF和MT1-MMP的表达 |
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| 引用本文： | 姚芳,闫喆,史永红,郝军,段惠军.高糖刺激下大鼠肾小球系膜细胞CTGF和MT1-MMP的表达[J].中国组织化学与细胞化学杂志,2009,18(1). |
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| 作者姓名： | 姚芳 闫喆 史永红 郝军 段惠军 |
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| 作者单位： | 河北医科大学病理学教研室,石家庄,050017 |
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| 摘 要： | 目的观察高糖刺激的大鼠肾小球系膜细胞结缔组织生长因子(CTGF)和膜型基质金属蛋白酶-1(MT1-MMP)的动态变化,探讨糖尿病肾病(DN)的发病机制。方法体外培养的大鼠HBZY-1肾小球系膜细胞分为正常糖对照组,高糖组和甘露醇高渗对照组,采用RT-PCR及Western印迹法分别检测CTGF和MT1-MMP的mRNA及蛋白的表达,用酶联免疫吸附法(ELISA)检测培养上清中IV型胶原的含量。结果与对照组相比,高糖组各时间点系膜细胞CT-GF表达明显上调,IV型胶原的分泌增加,且二者随时间持续增高;而MT1-MMP的表达则随时间呈明显下降趋势。结论高糖可诱导肾小球系膜细胞CTGF表达增加,同时抑制MT1-MMP的表达,二者可能参与DN中细胞外基质(ECM)代谢失衡过程。
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| 关 键 词： | 糖尿病肾病 肾小球系膜细胞 结缔组织生长因子 膜型基质金属蛋白酶-1 |
EXPRESSIONS OF CTGF AND MT1-MMP IN RAT GLOMERULAR MESANGIAL CELLS UNDER HIGH-GLUCOSE MEDIUM |
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| Yao Fang,Yan Zhe,Shi Yonghong,Hao Jun,Duan Huijun.EXPRESSIONS OF CTGF AND MT1-MMP IN RAT GLOMERULAR MESANGIAL CELLS UNDER HIGH-GLUCOSE MEDIUM[J].Chinese Journal of Histochemistry and Cytochemistry,2009,18(1). |
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| Authors: | Yao Fang Yan Zhe Shi Yonghong Hao Jun Duan Huijun |
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| Institution: | Department of Pathology;Hebei Medical University;Shijiazhuang 050017;China |
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| Abstract: | Objective To observe the high-glucose induced dynamic changes of connective tissue growth factor (CTGF) and membrane-type 1 matrix metalloproteinase (MT1-MMP) in rat glomerular mesangial cells (GMCs).Methods Rat HBZY-1 GMCs were cultured and divided into 3 groups: normal concentration of D-glucose (NG) group, high concentration of D-glucose (HG) group and mannitol plus D-glucose (NG+M) group. The mRNA and protein expressions of CTGF and MT1-MMP were detected with semi-quantitative RT-PCR and Western blot. T... |
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| Keywords: | Diabetic nephropathy Glomerular mesangial cell Connective tissue growth factor（CTGF） Membrane-type 1 matrix metalloproteinase（MT1-MMP） |
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