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| SiRNA抑制柯萨奇B3病毒的复制和表达 | |
| 引用本文： | 刘松兰,魏全,刘正湘,刘曌宇.SiRNA抑制柯萨奇B3病毒的复制和表达[J].中国组织化学与细胞化学杂志,2008,17(6):529-533. |
| 作者姓名： | 刘松兰 魏全 刘正湘 刘曌宇 |
| 作者单位： | 1. 华中科技大学同济医学院同济医院超声影像科康复医学科,武汉,430030 2. 华中科技大学同济医学院同济医院超声影像科心内科,武汉,430030 3. 华中科技大学同济医学院同济医院超声影像科消化内科,武汉,430030 |
| 摘 要： | 目的 研究观察体外合成siRNA对培养HELA细胞中柯萨奇B3病毒（Coxsackievirus B3，CVB3）的影响。方法根据siRNA靶序列设计原则，针对编码CVB3病毒聚合酶、VP1蛋白和5’非编码区基因组，特异性地体外合成三对siRNA，同时合成一对与CVB基因组序列无关的阴性对照siRNA。利用脂质体转染进入Hela细胞，用CVB3感染培养HELA细胞，观察转染后HELA细胞病变；采用RT-PCR技术检测感染CVB3各组的病毒RNA；用免疫荧光技术检测各组CVB3蛋白的表达；并用培养细胞上清液再感染HELA细胞观察病毒滴度。结果针对CVB3病毒聚合酶的siR-NA能有效的抑制病毒的复制和CVB3蛋白的表达，并能抑制病毒的再感染；而针对VP1蛋白和5’非编码区的siRNA能部分抑制病毒的复制和CVB3蛋白的表达。结论我们设计合成针对编码CVB3病毒聚合酶基因组的siRNA能有效抑制CVB3病毒复制和表达。 |
| 关 键 词： | 柯萨奇病毒B3(CVB3) RNA干扰 沉默RNA |
SiRNA INHIBITs REPLICATION AND EXPRESSION OF COXSACKIEVIRUS B_3 |
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| Liu Songlan,We Quan,Liu Zhengxiang,Liu Zhaoyu.SiRNA INHIBITs REPLICATION AND EXPRESSION OF COXSACKIEVIRUS B_3[J].Chinese Journal of Histochemistry and Cytochemistry,2008,17(6):529-533. | |
| Authors: | Liu Songlan We Quan Liu Zhengxiang Liu Zhaoyu |
| Institution: | Liu Songlan,We Quan1,Liu Zhengxiang2,Liu Zhaoyu3(Department of Medical Ultrasound of Tongji Hospital,1Department of Rehabilitation Medicine of Tongji Hospital,2Department of Cardiology,Tongji Hospital,3Department of Division of Gestroenterology of Tongji Hospital,Huazhong University of Science , Technology,Wuhan,430030 China) |
| Abstract: | Objective To investigate the effects of synthetic siRNA in vitro on coxsackievirus B3(CVB3)replication and expression.Methods On the basis of the principle of target sequence of siRNA,we designed 3 homologous sequences of the genome of CVB3,which coded polymerase,VP1,and 5' untranslation region(5'UTR)respectively,and subsequently synthesized 3 siRNA chemically in vitro.We also designed a control siRNA that was independent of the genome of CVB3.Hela cells were infected with CVB3 after siRNA transfection by l... |
| Keywords: | Coxsackievirus B3 RNA interference SiRNA |
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