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慢性脑低灌注大鼠海马BDNF的表达与认知功能损害
引用本文:郑萍,章军建,刘汉兴,周艳.慢性脑低灌注大鼠海马BDNF的表达与认知功能损害[J].中国组织化学与细胞化学杂志,2003,12(4):407-410.
作者姓名:郑萍  章军建  刘汉兴  周艳
作者单位:武汉大学中南医院神经内科,武汉,430071
基金项目:国家自然科学基金 (30 2 70 4 83)
摘    要:目的 观察慢性脑低灌注大鼠认知功能损害与海马脑源性神经营养因子 (Brain derivedneurotrophicfactor,BDNF)表达的关系。方法 通过结扎大鼠双侧颈总动脉 ,制成慢性脑低灌注模型 ,分缺血 6周组和 4月组及相应假手术组 ,在相应时间点用三等份MG 2Y型迷宫法测定大鼠学习记忆能力。用免疫组化S P法检测 4组大鼠海马中BDNF的表达 ,在光镜下测其平均光密度。结果 显示手术组与假手术组相比学习记忆能力明显下降 ,慢性脑低灌注大鼠缺血 4月组与缺血 6周组相比学习记忆能力也下降 (P <0 0 5 )。缺血 4月组大鼠海马BDNF表达的平均光密度值与Y型迷宫实验正确次数间存在正相关 (r=0 782 5 ,P <0 0 5 )。结论 表明在慢性脑低灌注状态下 ,大鼠学习记忆能力随海马BDNF表达的下降而下降 ,内源性BDNF可能通过对突触传递和认知功能有内在保护作用。

关 键 词:慢性脑低灌注  认知功能  脑源性神经营养因子
修稿时间:2003年10月13

COGNITIVE IMPAIRMENT AND EXPRESSION OF BDNF IN RAT HIPPOCAMPUS AFTER CHRONIC CEREBRAL ISCHEMIA
Zheng Ping,Zhang Junjian,Liu Hanxing,Zhou Yan.COGNITIVE IMPAIRMENT AND EXPRESSION OF BDNF IN RAT HIPPOCAMPUS AFTER CHRONIC CEREBRAL ISCHEMIA[J].Chinese Journal of Histochemistry and Cytochemistry,2003,12(4):407-410.
Authors:Zheng Ping  Zhang Junjian  Liu Hanxing  Zhou Yan
Abstract:Objective To observe the relationship between the cognitive impairment and the expression of BDNF in the hippocampus of rats after chronic cerebral ischemia. Method An animal model was established through bilateral common carotid arteries occlusion (2-VO). 40 male Wistar rats were randomized into control and experimental groups. The experimental group was subdivided into 2 subgroups 6 with either weeks or 4 months of ischemia, and so was the control group. The Y-type maze was used to test the learning and memory behavior of rats. The brain sections were processed by immunohistochemical S-P method with an antibody against BDNF. Result The learning and memory impairment in rats was increased significantly in the experimental group, especially after 4 months of ischemia. A perfect negative correlation was found between the BDNF immunoreactivity and the number of correct runs in the maze in the 4-month subgroup of ischemia (r0.7825,P<0.05), but not in the 6-week subgroup.Conclusion The results suggest that the expression of BDNF decreases following cognitive impairment, and BDNF synthesized by the brain itself may play an important protective role in the synapse transmission or cognitive function of the brain.
Keywords:Chronic  cerebral ischemiaCognitive impairmentBDNF
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