食管鳞状细胞癌中血管生成拟态及MMP-1蛋白的表达

目的 研究人食管鳞状细胞癌(human esophageal squamous cell carcinoma,ESCC)中是否存在血管生成拟态(vasculogenic mimicry,VM)及其与(Matrix metalloproteinase-1,MMP-1)表达的关系,以及MMP-1过表达的临床意义.方法收集118例食管鳞状细胞癌的标本,每例均有完整的临床资料,利用CD31(Platelet endothelial cell adhesion molecule,PECAM-1)和PAS套染观察是否存在VM,对VM组和对照组进行MMP-1染色,分析VM与MMP-1表达的关系及MMP-1表达与临床病理学参数的关系.结果 118例食管鳞状细胞癌组织中有34例(28.81%)存在VM,有VM生成组的MMP-1过表达比例显著高于无VM组,两组之间的差异有统计学意义(P<0.05).有淋巴结转移组的MMP-1过表达的比例显著高于无淋巴结转移组(P<0.05);浸润至深肌层及外膜层的MMP-1过表达的比例显著高于浸润之粘膜层和浅肌层的(P<0.05);临床分期Ⅲ-Ⅳ期的MMP-1蛋白的过表达显著高于临床分期Ⅰ-Ⅱ期的(P<0.05).结论 食管鳞状细胞癌中存在VM,MMP-1的过表达可能促进VM形成;VM的存在和MMP-1过表达共同促进淋巴结转移和肿瘤浸润.

Vasculogenic mimicry in esophageal squamous cell carcinoma and the expression of matrix metalloproteinase-1

Objective To study whether vasculogenic mimicry （VM） exist in human esophageal squamous cell carcinoma （ESCC）, its relationship with matrix metalloproteinase-1 （MMP-1） and clinicopatho- logical parameters. Methods 118 specimens of primary ESCC and their clinical data were collected, and dual staining of CD34 and Periodic Acid-Schiff （PAS） were performed to validate the existence of VM in ES- CC. MMP-1 over-expression was then detected in both groups with and without VM to analyze the rela- tionship between VM and MMP-1 protein, and the correlation of MMP-1 and clinicopathological parameters of ESCC. Results VM existed in 34 （28.81%） of 118 cases,and the over-expression of MMP-1 in cases with VM was obviously higher than that without VM （P〈0.05）. The over-expression of MMP-1 protein in patients with lymph nodes metastasis was higher than that without it （P〈0.05）. The over-expression of MMP-1 protein in patients with invasion in deep muscular layer and adventitial layer was higher than that in mucosa and superficial muscular layer （P〈0.05）, and higher in Ⅲ -Ⅳgstages than in I - Ⅱ stage. Conclusion VM existed in ESCC and the over-expression of MMP-1 protein may promote VM formation, lymph node metastasis and invasion.