| 小鼠胚胎干细胞定向分化为神经干细胞过程中microRNA的变化 |
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| 引用本文： | 邢变枝,陈红,湛彦强,张苏明.小鼠胚胎干细胞定向分化为神经干细胞过程中microRNA的变化[J].中国组织化学与细胞化学杂志,2009,18(1). |
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| 作者姓名： | 邢变枝 陈红 湛彦强 张苏明 |
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| 作者单位： | 1. 华中科技大学附属同济医院神经内科,武汉,430030
2. 华中科技大学附属同济医院康复科,武汉,430030 |
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| 摘 要： | 目的用生物芯片技术分析胚胎干细胞定向分化为神经干细胞过程中microRNA(miRNA)的表达变化,筛选调控的分化的miRNA,研究分化调控机制。方法胚胎干细胞在含LIF培养基中培养3d后,采用经典5步培养方法定向诱导向神经干细胞分化,采用nestin作为神经干细胞标记进行鉴定,送检胚胎干细胞及神经干细胞,提取总RNA以及小分子RNA,经荧光标记后与miRNA基因芯片杂交,获得胚胎干细胞诱导前后miRNA表达谱。结果1)胚胎干细胞在含LIF培养过程中保持未分化状态,Oct-4、碱性磷酸酶表达阳性;2)经典五步法诱导胚胎干细胞定向分化为神经干细胞,nestin阳性细胞为85%;3)通过基因微阵列分析,有90个miRNA的改变显著,其中68个表达上调,22个表达下调。结论miRNA可能对胚胎干细胞定向分化为神经干细胞过程起到关键作用。
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| 关 键 词： | 胚胎干细胞 神经干细胞 分化 miRNA 生物芯片 |
microRNA EXPRESSION CHANGE DURING THE DIFFERENTIATION OF MOUSE EMBRYONIC STEM CELLS INTO NEURAL STEM CELLS |
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| Xing Bianzhi,Chen Hong,Zhan Yanqiang,Zhang Suming.microRNA EXPRESSION CHANGE DURING THE DIFFERENTIATION OF MOUSE EMBRYONIC STEM CELLS INTO NEURAL STEM CELLS[J].Chinese Journal of Histochemistry and Cytochemistry,2009,18(1). |
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| Authors: | Xing Bianzhi Chen Hong Zhan Yanqiang Zhang Suming |
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| Institution: | 1Department of Neurology;2Department of Rehabilitation Medicine;Tongji Hospital;Tongji Medical College;HUST;Wuhan 430030;China |
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| Abstract: | Objective To find change of microRNA (miRNA)that influences embryonic stem (ES) cells differentiation.Methods ES cells were cultured in a medium with LIF at 1000u/ml.Three days later we used the classical five-step culture to differentiate ES cells into neural stem cells. The neural stem cells were tested by immunocytology. After the total RNA of ES and neural stem cells were collected, the expression of miRNA was analysed using a microchip to find the difference.Results 1) ES cells kept undifferentiated in... |
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| Keywords: | miRNA |
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