| NDRG2在糖尿病小鼠和正常小鼠胰岛、心肌和肾中的表达 |
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| 引用本文： | 沈岚,胡晓兰,刘新平.NDRG2在糖尿病小鼠和正常小鼠胰岛、心肌和肾中的表达[J].中国组织化学与细胞化学杂志,2008,17(5):407-411. |
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| 作者姓名： | 沈岚 胡晓兰 刘新平 |
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| 作者单位： | 第四军医大学生物化学与分子生物学教研室,国家肿瘤生物学重点实验室,西安,710032 |
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| 摘 要： | 目的该实验通过对糖尿病小鼠和正常小鼠胰岛、心肌和肾中NDRG2表达的研究，旨在阐明NDRG2在糖尿病小鼠和正常小鼠中的表达差异，为进一步明确分化相关基因NDRG2的功能提供依据。方法分离糖尿病小鼠和正常小鼠的胰腺、心肌和肾，并制备成石蜡切片，用抗NDRG2单克隆抗体，进行免疫组织化学染色（ABC法），从蛋白质水平观察NDRG2的表达情况。统计阳性细胞数，用统计学方法，判断糖尿病小鼠和正常小鼠中NDRG2的表达有无差别。结果免疫组织化学染色显示：①胰腺中NDRG2特异表达在胰岛细胞胞浆，相对正常小鼠而言Ⅱ型糖尿病小鼠胰岛中NDRG2表达略增强，Ⅰ型糖尿病小鼠胰岛中NDRG2表达略减弱；②心脏中NDRG2特异表达在心肌细胞胞浆，Ⅱ型糖尿病小鼠心肌细胞中NDRG2分布局限，正常小鼠心肌细胞中NDRG2分布均匀；③肾脏中NDRG2特异表达在肾小管上皮细胞的细胞浆，Ⅰ型糖尿病小鼠中肾小管上皮细胞出现空泡样变性。结论NDRG2在糖尿病小鼠和正常小鼠胰腺、心肌和肾中的表达差异提示NDRG2作为分化相关基因可能参与糖尿病的致病机制。
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| 关 键 词： | NDRG2 糖尿病小鼠 胰腺 心脏 肾 免疫组织化学 |
EXPRESSION OF NDRG2 IN PANCREAS,HEART AND KIDNEY OF DIABETIC AND NORMAL MICE |
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| Shen Lan,Hu Xiaolan,Liu Xinping.EXPRESSION OF NDRG2 IN PANCREAS,HEART AND KIDNEY OF DIABETIC AND NORMAL MICE[J].Chinese Journal of Histochemistry and Cytochemistry,2008,17(5):407-411. |
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| Authors: | Shen Lan Hu Xiaolan Liu Xinping |
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| Institution: | Shen Lan,Hu Xiaolan,Liu Xinping(Department of Biochemistry , Molecular Biology,National Key Laboratory of Cancer Biology,The Fourth Military Medical University,Xi\'an 710032,China) |
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| Abstract: | Objective To explore the expression features of NDRG2 in the pancreas,heart and kidney of diabetic and normal mice,and to investigate the differences in NDRG2 distribution between diabetic and normal mice,so as to provide some clues to the biological functions of the differentiation-related gene NDRG2.Methods The pancreas,heart and kidney tissues from diabetic and normal mice were dissected and embedded with paraffin.Serial sections were cut and mounted on glass slides.The immunohistochemical assay(ABC meth... |
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| Keywords: | NDRG2 |
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