Genistein对腺样囊性癌cyclinB1蛋白表达和细胞增殖周期的影响

目的研究酪氨酸蛋白激酶抑制剂genistein抑制人涎腺腺样囊性癌(SACC-83)细胞生长与cyclin B1蛋白表达和细胞增殖周期的关系.方法 MTT法测定genistein对SACC-83细胞体外生长的作用;流式细胞仪测定细胞增殖周期;Western Blot技术检测cyclin B1和Cdk1蛋白,并利用电泳凝胶成像分析软件对其结果进行量化分析;采用SPSS11.5统计软件对结果进行统计学分析.结果 Genistein对SACC-83细胞生长有抑制作用,且当其作用到一定时间达到一定的浓度后,该作用与剂量及时间呈依赖关系;Genistein作用的细胞与对照细胞比较,G0/G1期细胞数减少, G2/M期细胞数增多,cyclin B1和Cdk1蛋白水平降低.结论 Genistein对SACC-83细胞生长的抑制作用与其调节cyclin B1和Cdk1蛋白表达和细胞增殖周期有关.

EFFECTS OF GENISTEIN ON CYCLIN B1 PROTEIN EXPRESSION AND CELL CYCLE IN ADENOID CYSTIC CARCINOMA CELL LINE

Objective To investigate the growth inhibiting effect of genistein, a tyrosine protein kinase inhibitor, on the human salivary adenoid cystic carcinoma SACC-83 cell line in vitro, and its effects on the expression of cyclin B1 protein and the cell cycle. Methods The effects of genistein on the growth of SACC-83 cells in vitro were measured with MTT assay; the cell cycle was detected with flow cytometry; the expressions of cyclin B1 and Cdkl proteins were measured with Western blotting, and the results were quantitatively analyzed by FluorChem V2.0 software; and the results were statistically analyzed by SPSS11. 5 software. Results When treated with genistein of certain concentration for certain period of time, the growth of SACC-83 cells was inhibited in a dose- and time-dependant manner, and genistein-treated SACC-83 cells were arrested in the G2/M phase and had lower contents of cyclin B1 and Cdkl proteins compared with the control group. Conclusion The growth inhibiting effect of genistein on SACC-83 cells may be associated with the regulations of genistein on the cyclin B1 and Cdkl protein expressions and the cell cycle.