人乳腺癌细胞p53和bcl-2蛋白的表达状况及与激素受体的关系

为了进一步证明 p5 3和 bcl- 2癌基因蛋白的反向关系 ,我们用 SP法进行免疫细胞化学染色 ,观察了 p5 3、 bcl- 2、雌激素受体 (estrogen receptor,ER)、孕激素受体 (progesterone receptor,PR)在人乳腺癌细胞系 MCF7和 MDA- MB2 31中的表达情况 ,并应用图像分析系统对其阳性反应物进行定量。结果 :MCF7细胞表达 ER和 PR,而 MDA- MB2 31细胞不表达。二个细胞系均表达 p5 3,但 MCF7光密度 (OD)值为 0 .10 0 9± 0 .0 14,而 MDA- MB2 31细胞为 0 .16 78± 0 .0 42 ,后者明显高于前者 (P<0 .0 0 0 1)。二系细胞均可见到 bcl- 2阳性物质 ,但 MCF7表达的 OD值为 0 .10 45± 0 .0 2 0 8,而 MDA- MB2 31细胞仅为 0 .0 5 2 5± 0 .0 113(P<0 .0 0 0 1)。表明 bcl- 2的表达与 ER及 PR的存在有很强的相关性 ,并且与 p5 3的表达呈明显的相反关系

EXPRESSION OF p53 AND bcl-2 PROTEIN IN HUMAN BREAST CARCINOMA CELL LINE MCF7 AND MDA-MB231, AND ITS RELATIONSHIP TO HORMONE RECEPTORS

In order to prove the adverse relationship between p53 and bcl-2oncogene protein, the expressions of p53, bcl-2, estrogen receptor(ER) and progesterone receptor(PR) were determined in human breast carcinoma cell lines MCF7 and MDA-MB231 with immunocytochemical method, and quantitatively measured by image analysis system. In both of two lines there were the expressions of p53 and bcl-2 proteins. However, the optical density (OD) value of p53 protein exptression was significantly higher in the MDA-MB231 (0.1678±0.042) than that in the MCF-7 (0.1009±0.014) (P＜0.0001). But the OD value of bcl-2 protein expression was very lower in the MDA-MB231 (0.0525±0.0113) than that in the MCF-7 (0.1045±0.0208) (P＜0.0001). These data suggest that bcl-2 protein maybe correlated with ER and PR strongly, and adversely correlated with p53 protein.