五种酶组织化学方洁显示大鼠海马微血管的比较

本文对１０例成年Ｗｉｓｔａｒ大鼠海马，应用过氧化物酶二氨基联苯胺（ＤＡＢ）法、碱性磷酸酶（ＡＩＰ）、镁离子激活的三磷酸腺苷酶（Ｍｇ（２＋）－ＡＴＰａｓｅ）、钙离子激活的三磷酸腺昔酶（Ｃａ（２＋）－ＡＴＰａｓｅ）和５’－核苷酸酶（５’－Ｎａｓｅ）等酶组织化学方法显示其微血管，并应用体视学方法测算，比较上述方法显示微血管的效果，结果表明：ＤＡＢ法显示微血管的效果最好，ＡＩＰ法次之，Ｍｇ（２＋）－ＡＴ－Ｐａｓｅ法再次之。大鼠海马微血管Ｃａ（２＋）－ＡＴＰａｓｅ呈弱阳性，５‘－Ｎａｓｅ呈阴性。ＤＡＢ法和Ｍｇ（２＋）－ＡＴＰａｓｅ法分别适宜作微血管长度密度和血管直径的定量分析。

A COMPARATIVE STUDY OF FIVE ENZYMIC HISTOCHEMICAL METRODS ON SHOWING MICROVESSEL IN RAT HIPPOCAMPUS

To compare the effect of five enzymic histochemical methods, namely, peroxidase diaminobenzidine (DAB) method, alkaline phosphatase (AlP), Mg2+--ATPase, Ca2+--ATPase and5'-nucleotidase (5'--Nase) on showing microvessels in rat hippocampus, the hippocampal microvessels of 10 adult Wistar rats were observed by stereological analysis. We found that theDAB method occupied the first place on showing microvessel, followed by AlP method andMg2---ATPase method. The hippocampal microvessel. were weak positive by Ca2+--ATPasestaining and negative by 5'--Nase staining. DAB and Mg2+--ATPase method seemed to be fitfor quantitative analysis of microvessel length density and diameter respectively.