P53和PCNA的表达与大肠癌关系的免疫组织化学研究

本研究采用免疫组织细胞化学ＡＢＣ法对６２例大肠癌区、癌移行区及癌旁区组织内Ｐ５３和ＰＣＮＡ蛋白进行检测。结果表明，Ｐ５３蛋白在大肠癌区阳性率占６１．３％（３８／６２）、ＰＣＮＡ蛋白阳性率占７１．０％（４４／６２）、阳性结果均位于癌细胞核内，Ｐ５３蛋白阳性的癌细胞，少部分为核浆型。Ｐ５３及ＰＣＮＡ蛋白联合表达与大肠癌发生及发展相关密切，并互相协同致癌，对大肠癌分化程度和Ｄｕｋｅｓ分期影响较大，为大肠癌病变研究提供了分子生物学信息，对手术切除缘范围和评估大肠癌转移和预后有一定参考价值。

IMMUNOHISTOCHEMICAL STUDY ON RELATIONSHIP OF EXPRESSION OF P53 AND PCNA IN COLONIC CARCINOMA

p53 and PCNA protein was detected by immunohistochemical ABC method in coloniccarcinoma tissue , the transitional district tissue of carcinolna and the surrounding tissues ofcarcinoma in 62 cases. The results manifest that the positive rate of p53 protein was 61. 3%(38/62) in colonic carcinoma tissue and in PCNA protein it accounted for 71. 0% (44/62).The positive product was all in the nuclear of carcinomatous cells. A small number of carcinomatous cells with positive p53 protein belonged to nucleocytoplasmic type. The allied expression of p53 and PCNA proteins involved in the intertelation of generation and development of carcinoma. The two proteins might cause carcinoma in coordination, which had agreat influence in the differentiation extent md Duke's staging in colonic carcinoma, Thisstudy provides a biomolecular basis in research of celonic carcinoma, a definite value of neference in resected limits of operation and the evaluation of metastasis and prognosis ofcolonic carcinoma.