正常豚鼠耳蜗核一氧化氮合酶阳性神经元的投射

本文采用辣根过氧化物酶（ＨＲＰ）逆行追踪技术结合硫辛酰胺脱氨酸（ＮＡＤＰＨ－ｄ）组织化学方法，研究正常豚鼠耳蜗核一氧化氮合酶（ＮＯＳ）阳性神经元的上行投射特点。探讨耳蜗核ＮＯＳ阳性神经元在听觉信号传递中的可能作用。结果表明，一侧上橄榄复合体加压注射ＨＲＰ后，两侧耳蜗核均出现ＨＲＰ标记细胞，同侧耳蜗核ＮＯＳ－ＨＲＰ双标细胞较多占８２．６３％，并可见ＨＲＰ阳性纤维和终末包绕ＮＯＳ阳性胞体，对侧耳蜗核ＮＯＳ－ＨＲＰ双标细胞相对较少，仅占１４．８７％。一侧下丘加压注入ＨＲＰ后两侧耳蜗核均无ＨＲＰ－ＮＯＳ双标细胞。结果提示，耳蜗核ＮＯＳ阳性神经元向上橄榄复合体投射，可能具有调节听觉声信号传递的作用

PROJECTION OF POSITIVE NOS NEURONS IN COCHLEAR NUCLEUS OF NORMAL GUINEA PIGS

This experiment adopted horse radish peroxidase (HRP) retrograde tracing technique and NADPH diaphorase histochemistry method to study ascending projection of positive NOS neurons in cochlear nucleus of normal guinea pigs and the possible role of positive NOS neurons in transmitting auditory signals. The results showed that HRP labelled cells appeared in both cochlear nuclei after pressure injection of HRP into one upper olivary complex with 82 63% of NOS HRP double labelled cells in homolateral cochlear nucleus. Positive NOS cell bodies were wrapped by positive HRP fibres and terminals. No HRP NOS double labelled cells were found in both cochlear nuclei after pressure injection of HRP into one lower colliculous. These suggested that positive NOS neurons in cochlear nucleus might project to upper olivary complex and might be involved in transmitting auditory signals.