ABPS诱导单核细胞HLA-DR表达

目的研究牛膝多糖(ABPS)在体外激活单核细胞的效应.方法采集健康成人抗凝血,用贴壁法分离单核细胞,调节细胞数接种到24孔板.在370 C,5%CO2条件下,以同一时间不同ABPS浓度或同一ABPS浓度不同时间培养,用流式细胞术和电镜检测单核细胞的激活.结果 ABPS诱导单核细胞超微结构和HLA-DR的改变.结论 ABPS上调HLA-DR表达并能激活单核细胞.

ABPS-INDUCED HLA-DR EXPRESSION IN MONOCYTES

Objective To investigate the activating effect of Achyrathes bidentata polysaccharides (ABPS) on mononuclear cells in vitro.Method Heparinized blood was collected from adults. The monocytes were separated by the anchoring technique. Freshly isolated in 24-well flat-bottom plates, the cells were cultured with serially diluted ABPS-RPMI1640 for identical time or with the same ABPS-RPMI1640 for different periods at 37℃ in 5% CO2. The activation of the cells was assayed by electron microscopy and flow cytometry. Result Monocytes cultured with ABPS underwent substaintial changes in the cell ultrastructure, and the expression of HLA-DR was up-regulated in these cells in significant dose- and time-dependent manner. Conclusion ABPS can induce the expression of HLA-DR in the monocytes, and thereby activate them.