胃癌组织中幽门螺杆菌L型感染与淋巴管形成关系的研究

目的 研究胃癌组织中D2-40、LYVE-1标记的微淋巴管密度(LVD)、血管内皮生长因子受体(VEGFR-3)表达与幽门螺杆菌L型(helicobacter pylori L-form,Hp-L型)感染之间的关系.方法 应用革兰染色和免疫组化SP法检测80例胃癌组织和25例对照组的Hp-L型感染,同时用免疫组化SP法检测上述组织的LVD值和VEGFR-3的表达,分析Hp-L型与LVD以及VEGFR-3表达的关系结果 胃癌组织中革兰染色L型检出阳性率为67.5％；免疫组化Hp-L型抗原表达阳性率为65％,两种方法检测同时阳性的病例50例,占62.5％.胃癌组的Hp-L型阳性率、LVD及VEGFR-3表达阳性率均高于对照组(P＜0.01)；胃癌组中Hp-L阳性组的LVD值和VEGFR-3表达阳性率高于Hp-L阴性组.LVD与胃癌淋巴结转移具有一定关系.结论 Hp-L型感染与胃癌的发生、发展密切相关,Hp-L型可能是肿瘤淋巴管生成的重要促进因子,影响胃癌的侵袭和转移.

Relation between helicobacter pylori L-form infection and iymphangiogenesis in gastric carcinoma

Objective To investigate the correlation between infection of helicobacter pylori L-form and lymphangiogenesis of gastric carcinoma. Methods Hp-L was examined in 80 patients with gastric carcinoma and 25 controls by Gram staining and immunohistochemical SP method.The latter method was also used to detect the VEGFR-3 expression and to measure LVD marked by D2-40 and LYVE-1.Their relationship with clinicopathologic factors were analyzed. Results The positive rates of Hp-L in gastric carcinoma was 67.5% by Gram staining,which was similar to the positive rate 65% by immunohistochemical staining.Hp-L infection rate,LVD and VEGFR-3 were much higher in gastric carcinoma than in the control group（P0.01）.The LVD and VEGFR-3 in the Hp-L positive group of gastric carcinoma were significantly higher than in the Hp-L negative group.LVD was correlated with lymph node metastasis. Conclusion Hp-L infection is correlated with the occurrence of gastric carcinoma.Hp-L infection may be an important promoting factor in lymphangiogenesis and correlated to invasion and metastasis of gastric carcinoma.