| 大鼠脂肪基质细胞成脂分化及慢病毒感染的实验研究 |
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| 引用本文： | 刘莉,马爽,顾海伦,任亚浩,杨军.大鼠脂肪基质细胞成脂分化及慢病毒感染的实验研究[J].中国组织化学与细胞化学杂志,2010,19(4):327-330. |
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| 作者姓名： | 刘莉 马爽 顾海伦 任亚浩 杨军 |
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| 作者单位： | 1. 中国医科大学公共卫生学院营养与食品卫生学教研室,沈阳,110001
2. 中国医科大学附属盛京医院骨外科,沈阳,110004 |
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| 摘 要： | 目的诱导大鼠脂肪基质细胞成脂分化,观察慢病毒感染效果。方法大鼠脂肪基质细胞培养至第3代后,间接免疫荧光法鉴定细胞表面抗原CD44,并采用MTT法绘制生长曲线;第3代基质细胞成脂诱导分化为脂肪细胞,油红O染色法鉴定,并行慢病毒感染。结果第3代脂肪基质细胞表面抗原CD44表达呈阳性;细胞生长曲线呈"S"形。细胞经成脂诱导分化剂诱导10d后,胞内有大量脂滴形成,脂滴大小不等。油红O染色显示脂滴被染成红色;携带GFP报告基因的慢病毒可感染成熟脂肪细胞,感染率约为80%,且细胞被感染后状态良好。结果 慢病毒可高效感染由大鼠脂肪基质细胞成脂诱导分化成的脂肪细胞,为研究脂肪细胞的基因功能、相关疾病的基因治疗提供了一个有效工具。
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| 关 键 词： | 脂肪基质细胞 成脂分化 慢病毒 感染 |
EXPERIMENTAL STUDY ON ADIPOGENIC DIFFERENTIATION OF ADIPOSE-DERIVED STROMAL CELLS AND LENTIVIRUS INFECTION IN RATS |
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| Liu Li,Ma Shuang,Gu Hailun,Ren Yahao,Yang Jun.EXPERIMENTAL STUDY ON ADIPOGENIC DIFFERENTIATION OF ADIPOSE-DERIVED STROMAL CELLS AND LENTIVIRUS INFECTION IN RATS[J].Chinese Journal of Histochemistry and Cytochemistry,2010,19(4):327-330. |
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| Authors: | Liu Li Ma Shuang Gu Hailun Ren Yahao Yang Jun |
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| Institution: | Liu Li1,Ma Shuang1,Gu Hailun2,Ren Yahao1,Yang Jun1(1Department of Nutrition and Food Hygiene,School of Public Health,China Medical University,Shenyang 110001,2Division of Orthopedics,Shengjing Hospital,Shenyang 110004,China) |
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| Abstract: | Objective To induce adipogenic differentiation of rat adipose-derived stromal cells and to observe differentiated adipocytes infected by lentivirus.Methods Cell surface antigen CD44 was identified in rat adipose-derived stromal cells of generation 3 by indirect immunofluorescence miethod,and MTT assay showed the cell growth curve.Then we induced adipogenic differentiation.Oil red O staining were identified Adipocytes differentiated from adipose-dirived stromal cells were identified by oil red O staining,and... |
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| Keywords: | Adipose-derived stromal cell Adipogenic differentiation Lentivirus Infection |
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