白介素-4和白介素-6在慢性阻塞性肺病肺组织中的表达

为了探讨白介素 - 4(Interleukin- 4,IL- 4)和白介素 - 6 (Interleukin- 6 ,IL- 6 )在慢性阻塞性肺病 (COPD)患者肺组织中的表达变化及其在 COPD发病机制中的作用 ,45例因肺癌而行肺叶切除术患者的肺组织 ,根据有无 COPD及吸烟史分为 COPD组、吸烟对照组及非吸烟对照组 (每组各 15例 )。用免疫组织化学法测定肺组织炎性细胞中 IL- 4和 IL- 6的表达。结果表明 :1.IL - 4和 IL - 6在 COPD组、吸烟对照组及非吸烟对照组患者肺泡局部浸润的炎性细胞中有表达 ,其中 COPD组IL- 4和 IL- 6的表达强度明显高于吸烟对照组及非吸烟对照组 (P<0 .0 5 ) ,但吸烟对照组及非吸烟对照组 IL- 4和 IL- 6表达强度无显著差别 (P>0 .0 5 )。 2 .三组中 IL - 4在肺组织中的表达强度与 1秒钟用力呼气容积占预计值百分比 (FEV1 % pred)值之间均无直线相关关系 (r=- 0 .0 93,0 .0 0 2 45和 - 0 .2 5 2 ,P>0 .0 5 ) ;仅在 COPD组肺组织中的 IL- 6表达强度与 FEV1 % pre值呈直线负相关关系 (r=- 0 .6 3119,P<0 .0 5 ) ,其余各组未发现这种负相关关系。 3.COPD组 IL- 4与 IL- 6的表达呈直线正相关关系 (r=0 .5 99,P<0 .0 1)。结果提示 :1.IL - 4在 COPD患者细支气管及肺泡炎性细胞中表达增强 ,说明 Th2 型细胞因子可能也在 COPD发病

EXPRESSION AND SIGNIFICANCE OF INTERLEUKIN-4 AND INTERLEUKIN-6 IN LUNG TISSUE OF PATIENTS WITH CHRONIC OBSTRUCTIVE PULMONARY DISEASE

In order to investigate the changes of interleukin 4 and interleukin 6 expression in chronic obstructive pulmonary disease (COPD) and their effect on the pathogenesis of COPD, lung specimens were obtained from 45 lung cancer patients by pneumectomy The 45 cases were divided into a COPD group, a smoking control group and a nonsmoking control group, according to the COPD diagnosis and smoking history Immunohistochemical method was applied to identify the expression of IL 4 and IL 6 in inflammatory cells of pulmonay tissues The result showed that: 1) IL 4 and IL 6 were expressed in the local in filtrated in flammatary cells in bronchioli and alveoli, and the expression of IL 4 and IL 6 higher in the COPD group was markedly than in the smoking and nonsmoking control groups ( P <0 05), but there was no distinct difference between the later two groups ( P >0 05) 2) No correlation in IL 4 and FEV1% was found in any of the groups negative correlation between IL 6 and FEV 1% pre was found in the COPD group (r=-0 63119, P <0 05), but not in other groups 3) The expression of IL 4 was positively correlated with the expression of IL 6 (r=0 599, P <0 01) in the COPD group The study indicates that: 1) the high expression of IL 4 in COPD suggested Th 2 type cytokine might also play an important role in the pathogenesis of COPD; and 2) The high expression of IL 6 might play an important role in the progress of airway obstruction, which might reflect, and aggravate the local inflammation.