原位PCR技术研究鼻咽癌组织中bcl-2／JH融合基因

为研究bcl-2／JH融合基因与鼻咽癌发生的关系，应用兰套式原位PCR技术对41例石蜡包埋鼻咽癌组织中bcl-2/JH融合基因进行检测。结果发现41例癌组织中bcl-2／JH融合基因阳性6例，阳性率为14.6%,bcl-2基因的两个热点断裂区mbr和mcr与免疫球蛋白重链JH形成的融合基因mbr-JH和mcr-JH各占50％。bcl-2／JH融合基因形成与癌组织学分级和转移间无明显关系（P＞0.05）。本表明bcl-2/JH融合基因与鼻咽癌发生可能有一定关系，其中mbr-JH和mcr-JH同为bcl-2基因在鼻咽癌的两个重要基因异常改变形式。

STUDY OF THE BCL-2/JH FUSION GENE IN NASOPHARYNGEAL CARCINOMA TISSUE BY IN SITU PCR TECHNIQUE

To study the correlation between bcl 2/JH fusion gene and the occurrence of nasopharyngeal carcinoma (NPC), bcl 2/JH fusion gene in 41 cases of paraffin embedded NPC was investigated using semi nested in situ PCR technique. The results showed that bcl 2/JH fusion gene was present in 6 cases (14 6%), in which three were positive for mbr JH and three were positive for mcr JH. There were no significant differences in bcl 2/JH fusion gene formation between pathological grade and metastasis ( P >0 05). The result suggested that bcl 2/JH fusion might be involved in tumorigenesis of nasopharyngeal epithelium in which mbr JH and mcr /JH were the two important aberrant changes in NPC.