| M16添加硫氧还蛋白过氧化物酶Ⅱ无法克服昆明小鼠早胚体外发育2-细胞阻滞 |
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| 引用本文： | 史河秀,林翠英,谢美容,张卫玉,吕俊杰,王世鄂.M16添加硫氧还蛋白过氧化物酶Ⅱ无法克服昆明小鼠早胚体外发育2-细胞阻滞[J].中国组织化学与细胞化学杂志,2009,18(1). |
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| 作者姓名： | 史河秀 林翠英 谢美容 张卫玉 吕俊杰 王世鄂 |
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| 作者单位： | 福建医科大学基础医学院人体解剖学与组织胚胎学系,神经生物学研究中心,福州,350004 |
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| 基金项目： | 福建医科大学科学研究发展基金 |
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| 摘 要： | 目的观察硫氧还蛋白过氧化物酶Ⅱ(Peroxiredoxin Ⅱ,PrxII)是否可以克服昆明(Kunming)小鼠早胚体外发育2-细胞阻滞。方法取昆明小鼠1-细胞胚置于含PrxII蛋白的M16培养液中培养,观察PrxII对昆明小鼠早胚发育潜能和2-细胞胚内活性氧自由基(reactive oxygen species,ROS)水平的影响;同时比较昆明和B6C3F1小鼠1-细胞胚在M16中各自的发育情况;激光扫描共聚焦显微镜分别检测比较昆明与B6C3F1小鼠体外培养2-细胞胚内ROS水平以及昆明小鼠体外培养与体内发育2-细胞胚内ROS水平。结果M16培养液中添加PrxII蛋白(1nmol/L和100nMol/L)可以明显降低昆明小鼠体外培养2-细胞胚内ROS水平(P<0.05),但不能克服昆明小鼠体外发育2-细胞阻滞;昆明小鼠1-细胞胚在M16中培养存在2-细胞阻滞现象,而B6C3F1小鼠无2-细胞阻滞现象;昆明小鼠体外培养2-细胞胚内ROS水平显著低于体内发育2-细胞胚(P<0.05),亦略低于B6C3F1小鼠体外培养2-细胞胚内ROS水平(P>0.05)。结论M16培养液中添加PrxII可以明显降低2-细胞胚...
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| 关 键 词： | 硫氧还蛋白过氧化物酶Ⅱ 2-细胞阻滞 早胚 活性氧自由基 小鼠 |
M16 MEDIA SUPPLEMENTED WITH PEROXIREDOXIN II CAN NOT OVERCOME 2-CELL BLOCK OF KUNMING MOUSE EMBRYOS IN VITRO |
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| Shi Hexiu,Lin Cuiying,Xie Meirong,Zhang Weiyu,Lu Junjie,Wang Shie.M16 MEDIA SUPPLEMENTED WITH PEROXIREDOXIN II CAN NOT OVERCOME 2-CELL BLOCK OF KUNMING MOUSE EMBRYOS IN VITRO[J].Chinese Journal of Histochemistry and Cytochemistry,2009,18(1). |
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| Authors: | Shi Hexiu Lin Cuiying Xie Meirong Zhang Weiyu Lu Junjie Wang Shie |
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| Institution: | Department of Human Anatomy;Histology and Embryology;Research Center of Neurobiology;School of Preclinical Medicine;Fujian Medical University;Fuzhou 350004;China |
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| Abstract: | Objective To observe the effects of peroxiredoxin Ⅱ(PrxII) on the development of Kunming mouse early embryos, and to investigate whether PrxII could overcome the 2-cell block of Kunming mouse early embryos.Methods After Kunming 1-cell embryos were cultured in M16 media supplemented with PrxII protein, the development potential of the early embryo development and the reactive oxygen species (ROS) level of 2-cell embryos in vitro were tested. The different development potential of Kunming and B6C3F1 1-cell em... |
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| Keywords: | Peroxiredoxin Ⅱ（PrxII） 2-Cell block Early embryo Reactive oxygen species Mouse |
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