Analysis of carbon metabolism in transgenic<Emphasis Type="Italic">Arabidopsis thaliana</Emphasis> transformed with the cyanobacterial sucrose phosphate synthase gene
(1) Plant Metabolism Research Center & Graduate School of Biotechnology, Kyung Hee University, 449-701 Suwon, Korea
Abstract:
To investigate the effects of sucrose-phosphate synthase (SPS) on carbon partitioning, transgenicArabidopsis plants transformed withSynechocystis SPS were constructed. The integration, copy number and expression level were confirmed by Southern and Northern blot analyses.
SPS activity in leaves from the transgenic and wild type plants was not significantly different. The level of sucrose and
starch in the leaves of transgenic plant were slightly decreased compared to wild type. The glucose and fructose contents
were increased up to two-fold compared to wild type during the light period. It is our speculation that the decreased sucrose
level of the transgenic plant might be caused by the high acid invertase.
These authors contributed equally to this work