Analysis of the Tissue-SpecificS RNase gene promoter associated with self-incompatibility in tomato (Lycopersicon peruvianum L.) |
| |
Authors: | Myung Hee Kim Dong Ill Shin Hee Sung Park Dal Ung Kim Il Kyung Chung |
| |
Institution: | (1) Faculty of Life Resources, Catholic University of Taegu Hyosung, 713-702 Kyungsan, Kyungbuk, Korea;(2) Department of Agronomy, Kyungpook National University, 702-701 Taegu, Kyungbuk, Korea |
| |
Abstract: | To understand the expression pattern of theS RNase gene in the floral tissues associated with self-incompatibility (SI), promoter region of S11 RNase gene was serially deleted and fused GUS. Five chimeric constructs containing a deleted promoter region of the S11 RNase gene were constructed, and introduced intoNicotiana tabacum using Agrobacterium-mediated transformation. Northern blot analysis revealed that the GUS gene was expressed in the style,
anther, and developing pollen of all stages in each transgenic tobacco plant The developing pollen expressed the same amount
of GUS mRNA in all stages in transgenic tobacco plants. In addition, histochemical analysis showed GUS gene expression in
vascular bundle, endothecium, stomium, and tapetum cells during pollen development in transgenic plants. From these results,
it is speculated that SI ofLycopersicon peruvianum may occur through the interaction ofS RNase expressed in both style and pollen tissues. |
| |
Keywords: | Lycopersicon peruvianum Self-Incompatibility (SI) S RNase gene promoter |
本文献已被 SpringerLink 等数据库收录! |
|