Evaluation of Viability,Shedding Pattern,and Longevity of Pollen from Genetically Modified (GM) Herbicide-tolerant and Wild-type Zoysiagrass (<Emphasis Type="Italic">Zoysia japonica</Emphasis> Steud.) |
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Authors: | Hong-Gyu Kang Tae-Woong Bae Ok-Cheol Jeong Hyeon-Jin Sun Pyung-Ok Lim Hyo-Yeon Lee |
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Institution: | (1) Subtropical Horticulture Research Institute, Cheju National University, Jeju, 690-756, South Korea;(2) Faculty of Biotechnology, Cheju National University, Jeju, 690-756, South Korea;(3) Department of Science Education, Cheju National University, Jeju, 690-756, South Korea |
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Abstract: | Knowledge about pollen viability is important when evaluating the risk of genetically modified (GM) plants. Here, staining
via iodine potassium iodide (IKI) or triphenyl tetrazolium chloride (TTC) could not distinguish between live and dead pollen
from Zoysia japonica. Therefore, to obtain a reliable assessment of such viability and longevity, we developed an optimum germination medium containing
20% sucrose and 50 ppm H3BO3. Pollen grains transferred to the germination medium at about 1000 hours had a germination rate of >90%. Pollen was most
predominantly shed at approximately 1000 hours, with viability declining to nearly 0% at 1200 hours. All germinability was
lost within 150 min when stored at 25°C. No significant difference was found between GM and non-GM plants in their pollen
viability or longevity. |
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Keywords: | |
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