Differential antioxidative responses of ascorbate-glutathione cycle enzymes and metabolites to chromium stress in green gram (Vigna radiata L. wilczek) leaves |
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Authors: | Thirupathi Karuppanapandian Pritam Bala Sinha Abdul Majeeth Kamarul Haniya Kumariah Mamoharan |
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Institution: | (1) Department of Plant Sciences, Centre of Potential in Genomic Sciences, School of Biological Sciences, Madurai Kamaraj University, 625 021 Madurai, India;(2) Department of Biotechnology, Srimad Andavan Arts and Science College, 620 005 Thiruvanaikoil, Tiruchirappalli, India |
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Abstract: | Chromium-induced antioxidative responses of ascorbate-glutathione cycle enzymes and metabolites in green gram(Vigna radiata L. Wilczek) leaves were investigated in both dose and time-dependent manners. Rapid uptake of Cr was observed immediately
after the start of treatment. Significant reduction was observed in leaf biomass under 300 μM Cr-treatment. Treatment with
300 μM Cr increases the content of hydrogen peroxide and Superoxide dismytase activity upto initial 96 h, and then gradually
declined to the basal level. Ascorbate peroxidase and guaiacol peroxidase activities were low in 300 μM Cr-treated leaves
during the first 96 h, but significantly increased therefore, suggesting that increased enzyme activities would be responsible
for the removal of H2O2. Catalase activities were always suppressed under Cr stress. Contents of reduced ascorbate and dehydroascorbate were significantly
decreased under 300 uM Cr-treatment. The reduced glutathione content decreased at early stages of Cr-treatment. However, it
was restored to the normal level as in controls thereafter. In contrast, the glutathione disulphide content showed a progressive
increase during the initial hours of Cr-treatment. The non-protein thiol content was shown to increase during the first several
hours, but it declines at later stages. The present results demonstrate that Cr-induced oxidative stress is an important component
of the plant’s reaction to toxic levels of Cr. |
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Keywords: | antioxidative systems Cr toxicity green gram non-protein thiol reactive oxygen species |
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