A complete sequence of the pGA1611 binary vector |
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Authors: | Sung?-Ryul?Kim Sichul?Lee Hong?-Gyu?Kang Jong?-Seong?Jeon Kyung?-Me?Kim Email author" target="_blank">Gynheung?AnEmail author |
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Institution: | (1) National Research Laboratory of Plant Functional Cenomics, Division of Molecular and Life Sciences, Pohang University of Science and Technology (POSTECH), 790-784 Pohang, Korea;(2) Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University, 449-701 Yongin, Korea |
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Abstract: | We report the nucleotide sequence of the binary vector pGA1611, which is used for the transformation of foreign DNA into rice.
This vector is 13,476 bp long. The 5577- bp T- DNA region consists of a 1987- bp ubiquitine promoter region, 45 bp for the
multiple cloning site, a 253- bpnos terminator region, and the 2045- bpCaMV35S- hph- T7 chimaeric gene. The vector backbone (7004 bp) carriesoriT,traJ,trfA,tetA,tetR, andoriV. An 892- bp RB region and the 489- bp LB region are also present The T- DNA possesses 15 unique sites, six of which are at
the multiple cloning site. This information will be valuable for cloning foreign DNA and modifying the vector. |
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Keywords: | Binary vector pGA1611 rice sequence transformation |
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