首页 | 本学科首页   官方微博 | 高级检索  
   检索      


A complete sequence of the pGA1611 binary vector
Authors:Sung?-Ryul?Kim  Sichul?Lee  Hong?-Gyu?Kang  Jong?-Seong?Jeon  Kyung?-Me?Kim  Email author" target="_blank">Gynheung?AnEmail author
Institution:(1) National Research Laboratory of Plant Functional Cenomics, Division of Molecular and Life Sciences, Pohang University of Science and Technology (POSTECH), 790-784 Pohang, Korea;(2) Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University, 449-701 Yongin, Korea
Abstract:We report the nucleotide sequence of the binary vector pGA1611, which is used for the transformation of foreign DNA into rice. This vector is 13,476 bp long. The 5577- bp T- DNA region consists of a 1987- bp ubiquitine promoter region, 45 bp for the multiple cloning site, a 253- bpnos terminator region, and the 2045- bpCaMV35S- hph- T7 chimaeric gene. The vector backbone (7004 bp) carriesoriT,traJ,trfA,tetA,tetR, andoriV. An 892- bp RB region and the 489- bp LB region are also present The T- DNA possesses 15 unique sites, six of which are at the multiple cloning site. This information will be valuable for cloning foreign DNA and modifying the vector.
Keywords:Binary vector  pGA1611  rice  sequence  transformation
本文献已被 SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号