Freezing of swamp buffalo semen

The French mini straw technique (Society I.M.V.) was used to preserve semen of Indonesian swamp buffalo (Bubalus bubalis) in a lactose based extender with and without the removal of seminal plasma. Extended semen with 60–70% motility before freezing showed 60 and 40–50% motility after thawing at 4°C for 5 and 180 min. respectively. Nine of 13 cows conceived to a single insemination with frozen semen. Neither post-thawing motility nor conception was improved by removing seminal plasma before freezing