Gas chromatographic and mass spectrometric analysis of conjugated steroids in urine |
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| Authors: | Jong Man Yoon Kyung Ho Lee |
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| Institution: | (1) Department of Marine Biomedical Science, College of Ocean Science and Technology, Kunsan National University, Kunsan City, 573-702 Jeollabuk-do, Korea;(2) Department of Environmental Engineering, Kyongju University, Kyongju City, 780-210 Kyongsangbuk-do, Korea |
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| Abstract: | This study was carried out qualitatively and quantitatively to investigate the presence and the concentrations of anabolic
steroids in urine collected from orally administered humans. Microanalysis of conjugated steroids by gas chromatography and
mass spectrometry (GC/MS) has been carried out. Following oral administration three major metabolites of anabolic steroid
drugs have been detected and partially characterized. The six steroids can be analysed at the same time in 17 min. The lower
detection limit was 10 ng/ml in 5 ml of urine. The conjugated steroids from urine were centrifuged to 2,430g for 10 min, the supernatant solution passed through Amberlite XAD-2 column and the steroids eluted fraction esterified by
using MSTFA and TMSI. The rate of metabolism and urinary excretion seem to be reasonably fast. |
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| Keywords: | Conjugated steroids gas chromatography mass spectrometry metabolite |
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