Spore and crystal formation inBacillus thuringiensis var.thuringiensis during growth in cystine and cysteine |
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Authors: | S Rajalakshmi Y I Shethna |
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Institution: | (1) Microbiology and Cell Biology Laboratory, Indian Institute of Science, 560012 Bangalore |
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Abstract: | The effect of the addition of different concentratons of cystine and cysteine on sporulation and parasporal crystal formation
inBacillus thuringiensis var.thuringiensis was studied. The effect was well pronounced when the cystine/cysteine additions were made after the stationary phase. Heat
stable spores and crystals were formed when the culture was provided with a low concentration of cystine/cysteine (0.05 per
cent w/v). At a moderate concentration of cystine or cysteine (0.15%), only heat labile spores were formed without the production
of the crystal. When the cystine/cysteine concentration was high (0.25%), spore and crystal formation were completely inhibited.
Partial reversal of inhibition of sporulation was brought about by sodium sulphate or Zinc sulphate and lead, copper, cadmium
or cobalt acetate at 0.2 mM or at 0.2% of sodium or potassium pyruvate, citrate, cisaconitate, oxalosuccinate, ∞ -keto-glutarate,
succinate, fumarate, malate, or oxalacetate. Glutamate (0.2%) overcame the inhibitory effect of cystine/cysteine completely.
The structural changes observed using phase contrast microscopy were dependent upon the concentration of cystine/cysteine. |
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Keywords: | Bacillus thuringiensis var thuringiensis cystine cysteine spore crystal reversal of inhibition |
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