Transgenic tobacco plants expressingBoRS1 gene fromBrassica oleracea var.acephala show enhanced tolerance to water stress |
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Authors: | Dongqin Tang Hongmei Qian Lingxia Zhao Danfeng Huang Kexuan Tang |
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Institution: | (1) School of Agriculture and Biology, Shanghai Jiaotong University, 201101 Shanghai, People’s Republic of China;(2) Plant Biotechnology Research Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, School of Agriculture and Biology, Shanghai Jiaotong University, 200030 Shanghai, People’s Republic of China |
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Abstract: | Water stress is by far the leading environmental stress limiting crop yields worldwide. Genetic engineering techniques hold
great promise for developing crop cultivars with high tolerance to water stress. In this study, theBrassica oleracea var.acephala BoRS1 gene was transferred into tobacco throughAgrobacterium- mediated leaf disc transformation. The transgenic status and transgene expression of the transgenic plants was confirmed
by polymerase chain reaction (PCR) analysis, Southern hybridization and semi-quantitative one step RT-PCR analysis respectively.
Subsequently, the growth status under water stress, and physiological responses to water stress of transgenic tobacco were
studied. The results showed that the transgenic plants exhibited better growth status under water stress condition compared
to the untransformed control plants. In physiological assessment of water tolerance, transgenic plants showed more dry matter
accumulation and maintained significantly higher levels of leaf chlorophyll content along with increasing levels of water
stress than the untransformed control plants. This study shows thatBoRS1 is a candidate gene in the engineering of crops for enhanced water stress tolerance. |
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Keywords: | BoRS1 gene dry matter accumulation leaf chlorophyll content relative water content tobacco water stress |
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