Randomly amplified polymorphic DNA-polymerase chain reaction analysis of two different populations of cultured Korean catfishSilurus asotus |
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Authors: | Jong-Man Yoon Gye-Woong Kim |
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Institution: | (1) Department of Marine Biomedical Science, College of Ocean Science and Technology, Kunsan National University, Kunsan City, 573-702 Jeollabuk-do, the Republic of Korea;(2) Department of Animal Science, College of Industry Science, Kongju National University, 340-800 Yesan-kun, the Republic of Korea |
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Abstract: | Genetic similarity and diversity of cultured catfishSilurus asotus populations collected from two areas in western Korea were examined using randomly amplified polymorphic DNA-polymerase chain
reaction (RAPD-PCR). Out of 20 random primers tested, 5 produced 1344 RAPD bands ranging from 8.2 to 13.6 polymorphic bands
per primer. The polymorphic bands in these populations ranged from 56.4% to 59.6%. Polymorphic bands per lane within populations
ranged from 4.9% to 5.3%. The similarity within the Kunsan population varied from 0.39 to 0.82 with a mean (± SD) of 0.56
± 0.08. The level of bandsharing values was 0.59 ± 007 within the catfish population from Yesan. The genetic similarity in
cultured catfish populations may have been caused because individuals from two populations were reared in the same environmental
conditions or by inbreeding during several generations. However, in view of bandsharing values, polymorphic bands and also
the specific major bands that were inter-population-specific, significant genetic differentiation between these populations
were present even if bandsharing (BS) values were somewhat numerically different. Therefore, the number of RAPD polymorphisms
identified in this study may be sufficient to permit estimating genetic similarity and diversity. However, in future, additional
populations, sampling sites and individuals will be necessary to make up for these weak points. |
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Keywords: | Bandsharing cultured catfish DNA diversity polymorphic bands RAPD-PCR |
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