Restriction enzyme digestion of heterochromatin inDrosophila nasuta |
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Authors: | P K Tiwari S C Lakhotia |
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Institution: | (1) Cytogenetics Laboratory, Department of Zoology, Banaras Hindu University, 221 005 Varanasi, India;(2) Present address: School of Studies in Zoology, Jiwaji University, 474 011 Gwalior, India |
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Abstract: | In situ digestion of metaphase and polytene chromosomes and of interphase nuclei in different cell types ofDrosophila nasuta with restriction enzymes revealed that enzymes like AluI, EcoRI, HaeIII, Sau3a and SinI did not affect Giemsa-stainability
of heterochromatin while that of euchromatin was significantly reduced; TaqI and SalI digested both heterochromatin and euchromatin
in mitotic chromosomes. Digestion of genomic DNA with AluI, EcoRI, HaeIII, Sau3a and KpnI left a 23 kb DNA band undigested
in agarose gels while withTaqI, no such undigested band was seen. TheAluI resistant 23 kb DNA hybridized insitu specifically with the heterochromatic chromocentre. It appears that the digestibility of heterochromatin region in genome
ofDrosophila nasuta with the tested restriction enzymes is dependent on the availability of their recognition sites. |
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Keywords: | Drosophila heterochromatin: restriction enzyme digestion |
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