Chromium uptake bySaccharomyces cerevisiae and isolation of glucose tolerance factor from yeast biomass |
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Authors: | Vlatka Gulan Zetic Vesna Stehlik-Tomas Slobodan Grba Lavoslav Lutilsky Damir Kozlek |
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Institution: | (1) Laboratory of Fermentation and Yeast Technology, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia;(2) Present address: Food Control Center, Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia |
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Abstract: | Fermentations with yeastSaccharomyces cerevisiae in semiaerobic and in static conditions with the addition of chromic chloride into the used molasses medium were analysed.
It was proved that the addition of optimal amounts of CrCl3 into the basal medium enhanced the kinetics of alcohol fermentations. The addition of 200 mg/l CrCl3 into the medium stimulated both the yeast growth and the ethanol production in all experimental conditions. On the other
hand, the results showed that Cr3+ ions were incorporated into yeast cells during fermentation. Under these conditions the accumulation of Cr3+ ions was performed by yeast cells during the exponential growth phase, and with enriched amounts of 30–45 (μg/gd.m. of cells.
Yeast biomass enriched with chromium ions was extracted with 01 mol/l NH4OH assuming that the extracts had the glucose tolerance factor (GTF). Then the extracts were passed through a gel-filtration
column in order to isolate and purify the GTF. The presence of GTF in the purified fractions was determined by measuring the
absorbance at 260 nm.
It is evident from the obtained results that the added purified fractions enhanced the rates of CO2 production as well as the glucose utilization during alcoholic fermentation. As expected, the enhancement of both rates depended
on the amounts of extracts added to the fermentation substrate. Thus, it is evident that purified extracts contained the GTF
compound, and that Cr3+ ions were bonded to the protein molecule. |
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Keywords: | Glucose tolerance factor Saccharomyces cerevisiae yeast enriched with chromium |
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