Cytokinin binding proteins from mammalian sera |
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Authors: | C Jayabaskaran P Senapathy T M Jacob |
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Institution: | (1) Department of Biochemistry and ICMR Centre for Cell Biology and Genetics, Indian Institute of Science, 560 012 Bangalore |
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Abstract: | A cytokinin-binding protein fraction was isolated from normal rabbit sera by affinity chromatography. The protein fraction
bound tritium labelled N6- (δ2-risopentenyl) adenosine and the order of inhibition of this binding by competing non-radioactive compounds was, N6-(δ2-isopentenyl) adenosine < N6-benzyIadenosine < zeatin-riboside > N6-(δ2-isopentenyl) adenine < kinetin riboside > adenosine. The protein fraction showed broad specificity, the prefered cytokinin
being N6-(δ2-isopentenyl) adenosine. This is the first report of the isolation of cytokinin binding proteins from mammalian sources. |
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Keywords: | N6-(δ 2-isopentenyl) adenosine binding protein rabbit sera |
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