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Cytokinin binding proteins from mammalian sera
Authors:C Jayabaskaran  P Senapathy  T M Jacob
Institution:(1) Department of Biochemistry and ICMR Centre for Cell Biology and Genetics, Indian Institute of Science, 560 012 Bangalore
Abstract:A cytokinin-binding protein fraction was isolated from normal rabbit sera by affinity chromatography. The protein fraction bound tritium labelled N6- (δ2-risopentenyl) adenosine and the order of inhibition of this binding by competing non-radioactive compounds was, N6-(δ2-isopentenyl) adenosine < N6-benzyIadenosine < zeatin-riboside > N6-(δ2-isopentenyl) adenine < kinetin riboside > adenosine. The protein fraction showed broad specificity, the prefered cytokinin being N6-(δ2-isopentenyl) adenosine. This is the first report of the isolation of cytokinin binding proteins from mammalian sources.
Keywords:N6-(δ  2-isopentenyl) adenosine  binding protein  rabbit sera
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