A method for studying histochemical reactions in intact human dentine with a polarizing incident light microscope |
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Authors: | Eruch B Fanibunda |
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Institution: | (1) C/2, Amerchand Mansion, 16. Madam Cama Road, 400 039 Bombay, India |
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Abstract: | Localization and distribution of non-specific esterases has been studied in intact human dentine, by reflected light microscopy.
The method of specimen preparation described here permits the visualization of optical sections in depth within the specimen
at high optical resolution. Non-specific esterase was found deposited as discrete bands across the tubules. or as droplets,
or as a diffuse microsomal variety in the dentinal tubules and in the interglobular spaces. It was possible to distinguish
the droplet variety from the microsomal variety, of esterase within the same tubule, by means of a novel optical method using
antiflex and differential interference contrast systems of reflected light microscopy. It was found that the coefficient of
reflection of dentine diminished gradually from the enamel to the pre-dentine and was inversely related to the scattering
of light in dentine. This scattering plays an important role in the formation of the image with reflected light microscopy.
The reflected light microscope offers an economically attractive alternative or a supplementary mode of microscopy to the
confocal scanning microscopes for studying intact dentine at varying depths. |
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Keywords: | Reflected microscopy dentine esterase tubules morphology |
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