Effect of light on nucleotide modifications in the transfer RNA of cucumber cotyledons |
| |
Authors: | M Putta Raju C Jayabaskaran |
| |
Institution: | (1) Department of Biochemistry, Indian Institute of Science, 560 012 Bangalore, India |
| |
Abstract: | The effect of light on nucleotide modifications in the tRNA of cucumber (Cucumis sativus L. var. Guntur) cotyledons was studied by chromatographic, electrophoretic and immunological methods. The tRNA from light-grown
tissue showed the absence of 2-methylguanosine and a decrease in the relative proportions of ribothymidine and cytokinin-active
ribonucleosides when compared to those produced from dark-grown tissue. On the other hand, a significant amount of one species
of 2′-O-methyldinucleotide was observed in the tRNA of light-grown tissue which was not detected in the dark-grown tissue.
Also, tRNA from light-grown tissue had higher levels of another species of 2′-O-methyldinucleotide. The results showed no
difference in the amounts of other modified nucleosides in tRNA between tissues grown under the two conditions. 2′-O-Methyl-l-methyladenosine,
a nucleotide modified both in the base and the ribose, apparently specific to plant tRNAs, has been found to be present in
the RNA of both light- and dark-grown tissues. These results on the variation in modified nucleotides suggest that light has
some role in nucleotide modification and, consequently, in cellular functions. |
| |
Keywords: | tRNA light modified nucleotides Cucumis sativus |
本文献已被 SpringerLink 等数据库收录! |
|