Inhibition of glutathione synthesis reduces chilling tolerance in maize |
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Authors: | Gábor Kocsy Peter von Ballmoos Marianne Suter Adrian Rüegsegger Ulrich Galli Gabriella Szalai Gábor Galiba Christian Brunold |
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Institution: | (1) Institute of Plant Physiology, University of Berne, Altenbergrain 21, 3013 Berne, Switzerland, CH;(2) Agricultural Research Institute of the Hungarian Academy of Sciences, 2462 Martonvásár, P.O.B. 19, Hungary, HU |
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Abstract: | The role of glutathione (GSH) in protecting plants from chilling injury was analyzed in seedlings of a chilling-tolerant
maize (Zea mays L.) genotype using buthionine sulfoximine (BSO), a specific inhibitor of γ-glutamylcysteine (γEC) synthetase, the first enzyme
of GSH synthesis. At 25 °C, 1 mM BSO significantly increased cysteine and reduced GSH content and GSH reductase (GR: EC 1.6.4.2)
activity, but interestingly affected neither fresh weight nor dry weight nor relative injury. Application of BSO up to 1 mM
during chilling at 5 °C reduced the fresh and dry weights of shoots and roots and increased relative injury from 10 to almost
40%. Buthionine sulfoximine also induced a decrease in GR activity of 90 and 40% in roots and shoots, respectively. Addition
of GSH or γEC together with BSO to the nutrient solution protected the seedlings from the BSO effect by increasing the levels
of GSH and GR activity in roots and shoots. During chilling, the level of abscisic acid increased both in controls and BSO-treated
seedlings and decreased after chilling in roots and shoots of the controls and in the roots of BSO-treated seedlings, but
increased in their shoots. Taken together, our results show that BSO did not reduce chilling tolerance of the maize genotype
analyzed by inhibiting abscisic acid accumulation but by establishing a low level of GSH, which also induced a decrease in
GR activity.
Received: 9 November 1999 / Accepted: 17 February 2000 |
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Keywords: | : Abscisic acid – Buthionine sulfoximine – Cysteine – Glutathione – Glutathione reductase – Zea |
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