Subcellular compartmentation of fructose 2,6-bisphosphate in oat mesophyll cells |
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Authors: | Manfred Steingraber William H Outlaw Jr Rüdiger Hampp |
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Institution: | (1) Institut für Biologie I der Universität Tübingen, Auf der Morgenstelle 1, D-7400 Tübingen, Federal Republic of Germany;(2) Present address: Biology I, Florida State University, 32306-3050 Tallahassee, FL., USA |
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Abstract: | Evacuolated mesophyll protoplasts from oat (Avena sativa L.) were fractionated by a membrane-filtration technique. This method of rapid quenching of metabolic reactions permitted estimation of the in-vivo pools of fructose 2,6-bisphosphate (Fru2,6bisP) in the cytosol, chloroplasts and mitochondria. Vacuolar Fru2,6bisP was calculated as the difference between control protoplasts and evacuolated ones. The results indicate that Fru2,6bisP is exclusively cytosol-located in oat mesophyll protoplasts. Assuming a cytosolic volume of about 2 pl per evacuolated protoplast, the cytosolic concentration there was 11 M if protoplasts were in darkness. Illumination of either control or evacuolated protoplasts resulted in a significant decrease in the Fru2,6bisP content within 5 min.Abbreviations EPs
evacuolated protoplasts
- Fru2,6bisP
fructose 2,6-bisphosphate
- PFP
fructose 6-phosphate kinase (pyrophosphate-dependent), EC 2.7.1.90
- PEPCase
phosphoenolpyruvate carboxylase, EC 4.1.1.31 |
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Keywords: | Avena (mesophyll protoplasts) Compartmentation Cytosol Fructose 2 6-bisphosphate Membrane filtration Protoplast evacuolated |
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