Purification and properties of an endospermic protein of maize associated with the Opaque-2 and Opaque-6 genes |
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Authors: | N Di Fonzo L Manzocchi F Salamini C Soave |
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Institution: | (1) Sezione Maiscoltura, Istituto Sperimentale per la Cerealicoltura, Bergamo, Italy;(2) Istituto Biosintesi Vegetali, C.N.R., Milano, Italy;(3) Max-Planck Institut für Züchtungsforschung, D-5000 Köln, Federal Republic of Germany;(4) Sezione di Bergamo, Istituto Sperimentale per la Cerealicoltura, Via Stezzano 24, J-24100 Bergamo, Italy |
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Abstract: | Maize endosperms accumulate during development a large amount of storage proteins (zeins). The rate of zein accumulation is under the control of several regulatory genes. Two of these, the opaque-2 and opaque-6 mutants, lower the zein level, thus improving the nutritional quality of maize meals. An endosperm protein of Mr 32 000 (b-32) appears to be correlated with the zein level. The b-32 protein is encoded by the opaque-6 gene which, in turn, is activated by opaque-2. We report the purification, amino-acid composition and peptide map of b-32 protein. Furthermore we demonstrate that the protein exists as a monomer likely located in the soluble cytoplasm. As a step towards the isolation of a complementary-DNA clone for b-32 protein, the purification of its corresponding mRNA is described.Abbreviations b-32
endosperm protein of Mr 32000
- cDNA
complementary DNA
- EDTA
ethylenediaminetetraacetic acid
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O2, O6
opaque 2, opaque-6 genes
- PMSF
phenylmethylsulfonylfluoride
- RSP
reduced soluble proteins
- SDS-PAGE
sodium dodecyl sulfate-polyacrylamide gel electrophoresis |
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Keywords: | Endosperm Mutant (opaque-2 opaque-6) Protein (b-32) Zea (storage protein) |
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