A reassessment of the binding of napthaleneacetic acid by membrane preparations from maize

Naphthalene-1-acetic acid (NAA) binding by membrane fractions derived from maize has been re-evaluated. Using a computer curve-fitting procedure only one major type of NAA binding, in terms of binding affinity, could be identified. Auxins, antiauxins and structurally related compounds have been tested for their competitive effect on NAA binding and the inhibitor constants for a number of these have been determined. Extracts from various plant species have been examined for their NAA binding ability, but all showed much less binding than maize leaf or coleoptile preparations. The possibility of the NAA binding by maize extracts being due to a true hormone receptor is discussed.Abbreviations BA benzoic acid - CPIB p-chlorophenoxyisobutyric acid - 2,4-D 2,4-dichlorophenoxyacetic acid - DCB 2,4-dichlorobenzoic acid - IAA indolyl-3-acetic acid - NAA napthalene-1-acetic acid - 2-NAA napthalene-2-acetic acid - NAOA napthalene-2-oxyacetic acid - PA phenylacetic acid - PU phenylurea - 2,4,5-T 2,4,5-trichlorophenoxyacetic acid - TIBA 2,3,5-triiodobenzoic acid