Axenic culture of meristem tips of Rosa multiflora

Summary Excised shoot apices (0.6 or 1.0 mm long) of Rosa multiflora were grown in axenic culture on defined media including auxins, gibberellic acid and cytokinins. In the presence of zeatin (10^-8–10^-7M) and 6-benzylaminopurine (10^-7–10^-6 M) apices grew into plantlets but two other cytokinins, kinetin and 6-(3-methylbut-2-enyl)aminopurien, were ineffective. Auxins or gibberellic acid were either inhibitory or had no effect on differentiation.