Herbicide-resistant sugarcane (Saccharum officinarum L.) plants by Agrobacterium-mediated transformation |
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Authors: | Gil A Enríquez-Obregón Roberto I Vázquez-Padrón Dmitri L Prieto-Samsonov Gustavo A De la Riva Guillermo Selman-Housein |
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Institution: | (1) Plant Division, Centre for Genetic Engineering and Biotechnology (CIGB), P.O. Box 6162, 10600 Havana, Cuba, CU |
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Abstract: | The presence of undesirable plants in sugarcane (Saccharum officinarum L.) plantations reduces crop yields. Using genetic engineering as a complement for traditional breeding methods it is possible
to introduce herbicide-resistant traits into Saccharum germplasm. Transgenic sugarcane plants resistant to phosphinothricine (PPT), the active compound of the commercial herbicide
BASTA were generated by Agrobacterium tumefaciens-mediated transformation. Meristematic sections of sugarcane were treated with anti-necrotic compounds to minimize oxidative
bursts and used as explants. Four transformation protocols were assessed and the transformation frequencies reached 10–35%.
The regeneration rate was high and did not appear to be affected by the transformation procedure. Southern blot analysis of
several transformed plants indicated the integration per genome of one or two intact copies of the bar gene which encodes PPT acetyltransferase and confers resistance to BASTA. The levels of BASTA resistance were evaluated under
greenhouse and small-plot conditions.
Received: 8 November 1997 / Accepted: 22 November 1997 |
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Keywords: | :Agrobacterium Antioxidative treatment BASTA Herbicide resistance Saccharum (transformation) Transgenic sugarcane |
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