Glyphosate inhibits the translocation of green fluorescent protein and sucrose from a transgenic tobacco host to<Emphasis Type="Italic"> Cuscuta campestris</Emphasis> Yunk. |
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Authors: | Talia?Nadler-Hassar Alexander?Goldshmidt Email author" target="_blank">Baruch?RubinEmail author Shmuel?Wolf |
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Institution: | (1) R.H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, 76100 Rehovot, Israel |
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Abstract: | The parasitic plant Cuscuta campestris is dependent on its host for water, assimilates and amino acids. It can be controlled by the herbicide glyphosate, which inhibits 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), resulting in shikimate accumulation. In this study, C. campestris was parasitic on transgenic tobacco plants expressing green fluorescent protein (GFP) in the phloem. Changes in 14C]sucrose and GFP accumulation in the parasite were used as indicators of the herbicides effect on translocation between the host and parasite. Host plants were treated with glyphosate 22 days after sowing. Shikimate accumulation in the parasite 1 day after glyphosate treatment (DAGT) confirmed EPSPS inhibition in C. campestris. No damage was visible in the host plants for the first 3 DAGT, while during that same time, a significant reduction in 14C]sucrose and GFP accumulation was observed in the parasite. Thus, we propose that the parallel reduction in GFP and sucrose accumulation in C. campestris is a result of a glyphosate effect on the parasites ability to withdraw assimilates from the host.Abbreviations CLSM Confocal laser-scanning microscope - DAGT Days after glyphosate treatment - DAS Days after sowing - EPSPS 5-Enolpyruvylshikimate-3-phosphate synthase - GFP Green fluorescent protein |
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Keywords: | Cuscuta 5-Enolpyruvylshikimate-3-phosphate synthase Green fluorescent protein Glyphosate Sucrose translocation (inhibition) |
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