Alternative formation of anthraquinones and lipoquinones in heterotrophic and photoautotrophic cell suspension cultures of Morinda lucida Benth. |
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Authors: | U Igbavboa H-J Sieweke E Leistner I Röwer W Hüsemann W Barz |
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Institution: | (1) Institut für Pharmazeutische Biologie, Rheinische Friedrich-Wilhelms-Universität, Nussallee 6, D-5300 Bonn 1, Federal Republic of Germany;(2) Lehrstuhl Biochemie der Pflanzen, Westfälische Wilhelms-Universität, Hindenburgplatz 55, D-4400 Münster, Federal Republic of Germany |
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Abstract: | Photoheterotrophic and photoautotrophic cell suspension cultures were raised from a callus tissue derived from a Morinda lucida Benth. plant (Rubiaceae). The cultures were characterized with regard to fresh weight, dry weight, cell number, pH, chlorophyll and quinoid natural products. The amount of lipoquinones (phylloquinone, -tocopherol, plastoquinone, ubiquinone) isolated from the photoautotrophic cultures matched the amount detected in an intact leaf. Anthraquinone glycosides which are found in the roots of Morinda plants were not present in the photoautotrophic culture. The photoheterotrophic culture contained only trace amounts of these pigments. Abundant anthraquinone synthesis was observed when photoautotrophic and photoheterotrophic suspension cultures were transferred into darkness, provided sucrose was present in the medium. Induction of synthesis of anthraquinone pigments coincided with a rapid disappearance of lipoquinones from the culture. Thus, in the suspension culture, photoautotrophy correlates with lipoquinone synthesis and heterotrophy correlates with anthraquinone synthesis. This reflects the situation in the intact plants where lipoquinones are chloroplast-associated whereas anthraquinones occur in the roots.Abbreviation HPLC
high-performance liquid chromatography |
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Keywords: | Anthraquinone Cell suspension culture (photoautotrophic photoheterotrophic heterotrophic) Lipoquinone Morinda |
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